A kind of breeding method of the hybrid embryo of Rhododendron splendor and Rhododendron big character
A technology of large-character Rhododendron and hybrid embryos, applied in the field of plant breeding, can solve the problems of hindering Rhododendron hybrid breeding, abortion of hybrid embryos, failure of seeds to germinate, etc., achieving good practicability and industrialization prospects, improving the germination rate, and shortening the breeding cycle. Effect
Inactive Publication Date: 2016-08-24
南京润禾生态农业发展有限公司
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Problems solved by technology
However, there are obstacles after fertilization in distant hybridization of rhododendrons, mainly manifested as abortion of hybrid embryos, inability to germinate seeds and albino seedlings in offspring, etc. This situation hinders the process of hybrid breeding of rhododendrons in my country. Embryo rescue research is rarely reported
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[0026] A method for breeding the hybrid embryos of Rhododendron splendens and Rhododendron magnanima, comprising: cultivating the immature hybrid embryos in the primary culture medium, transferring the sprouted seedlings formed after the primary culture to the secondary proliferation medium for cultivation, and culturing the seedlings obtained after the proliferation and culture The sprouts were inserted into the rooting medium for rooting culture.
[0027] 1) Primary culture
[0028] Harvest the capsules 3 months after pollination, scrape off the fluff on the surface of the capsules, place them on an ultra-clean workbench, disinfect with 75% ethanol for 30 seconds, rinse with sterile water for 3 times, then disinfect with 10% NaClO for 15 minutes, and disinfect with sterile water Rinse 5 times, dry in the air, cut open with a scalpel to take the seed embryos, and culture them on WPM medium for 120 days, wherein 50mg / L GA is added to the WPM medium 3 , 30g / L sucrose and 8g / L ...
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A disclosed breeding method for hybrid embryo of R. pulchrum and R. schlippenbachi comprises: 1) performing initial culture, namely, getting a hybrid embryo of R. pulchrum and R. schlippenbachi, culturing on WPM (Woody Plant Medium) for 120 days, so as to germinate a seedling; 2) performing secondary culture, namely, getting the seedling germinated in the initial culture medium, inoculating into Read medium for culturing, and changing the spinner bottle for one time every 40 days; and 3) performing rooting culture, namely selecting a single seedling with strong growth, and inoculating into a rooting medium for rooting culture. According to the breeding method for the hybrid embryo of R. pulchrum and R. schlippenbachi, immature hybrid embryo is subjected to initial culture and germinates a seedling, the seedling is subjected to propagation culture for obtaining a bud seedling, then the bud seedling is inoculated into a rooting medium, so that rooting culture is successively performed, the germination rate of the hybrid embryo of R. pulchrum and R. schlippenbachi is substantially improved, seedling growth is promoted, breeding period is shortened, and the method has extremely good practicability and industrialization prospect.
Description
technical field [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a method for breeding hybrid embryos of Rhododendron splendens and Rhododendron grandis. Background technique [0002] Rhododendron is a plant belonging to Rhododendronaceae, which has high ornamental, edible and medicinal value. There are about 542 species of rhododendrons in China, which is the distribution center of rhododendrons in the world, but most of the fine wild rhododendrons are still hidden in the mountains. The total number of cultivated species of rhododendrons in my country is less than 300. Foreign Rhododendron crossbreeding started earlier. As of 2004, 28,000 new Rhododendron varieties had been registered, and varieties of cold-resistant, large-flowered, early-flowered, late-flowered and fragrant-flowered varieties had been bred. Domestic rhododendron breeding started relatively late in the 1980s. As of 2004, only 34 varieties had been registe...
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IPC IPC(8): A01H4/00
Inventor 耿兴敏徐雁南杨君凯吴影倩王良桂
Owner 南京润禾生态农业发展有限公司