Tissue culture method for clitoria ternatea

A tissue culture and butterfly bean technology, applied in the field of plant tissue culture, can solve the problems of low seed germination rate and slow reproduction speed of butterfly bean, and achieve the effects of neat and consistent reproduction of offspring, fast reproduction speed and high transplant survival rate.

Inactive Publication Date: 2015-01-28
FLOWER RES INST GUANGXI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a tissue culture method of butterfly pea, wh

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] On July 2, 2012, choose plump butterfly pea seeds, rinse with tap water, sterilize with 75% alcohol for 40 seconds, rinse with sterile water for 3 times, then sterilize with 0.1% mercuric chloride for 10 minutes, and rinse with sterile water Germinate after 3 times. Seeds were soaked in sterile water at 50°C for 15 minutes for germination, and then placed at room temperature for 24 hours. Then they were inserted into the modified MS medium and placed in a tissue culture room with a temperature of 28±2°C and light for 12 hours a day.

[0025] After a period of observation, statistics were carried out on July 17: the seeds began to germinate on the 5th day, the pollution rate was 0, and the germination rate was 36.7%. On July 25, the epicotyls, hypocotyls, and cotyledons of the seedlings after the germination (the cotyledons had been unfolded) were cut into 5mm segments, and inoculated on the improved MS medium respectively.

[0026] After a period of observation, it wa...

Embodiment 2

[0030] On July 2, 2012, select plump butterfly pea seeds, rinse with tap water, sterilize with 70% alcohol for 30 seconds, rinse with sterile water for 4 times, then sterilize with 0.1% mercuric chloride for 10 minutes, and rinse with sterile water Germinate after 4 times. Seeds were soaked in sterile water at 60°C for 15 minutes for germination, and then placed at room temperature for 24 hours. Then sow them in sterilized moist sand (during the germination process, the sand should be kept moist, but there should be no accumulated water), and placed in the tissue culture room with a temperature of 28±2°C and 12 hours of light per day.

[0031] After a period of observation, statistics were made on July 17: the seeds began to germinate on the 7th day, the pollution rate was 0, and the germination rate was 27.27%. On July 25, the epicotyls, hypocotyls, and cotyledons of the seedlings after the germination (the cotyledon had been unfolded) were cut into 10 mm segments, and inocu...

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PUM

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Abstract

The invention discloses a tissue culture method for clitoria ternatea. The method comprises the following steps: sterile seed germination, primary culture, callus differentiation, rooting culture, and acclimatization and transplanting. The method provided by the invention is used for the tissue culture for clitoria ternatea, and has the advantages of high reproduction speed, large reproduction coefficient, uniform progenies, and high transplanting survival rate.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture of butterfly pea. Background technique [0002] Butterfly bean (Clitoria ternatea L.), also known as blue butterfly, blue flower bean, butterfly flower bean, is a plant of the genus Clitoria (Papilionaceae) and is native to India, Indonesia and other places. Widely cultivated in subtropical regions. Butterfly pea has a wide range of economic value. The flowers can be used to extract natural dyes; the tender clips can be eaten; In the configuration of garden plants and landscaping. [0003] At present, there are very few studies on butterfly pea, and domestic research fields mainly involve the simple description of some characteristics, as well as the research on seed germination and cultivation techniques. The germination rate of butterfly pea seeds is generally low, and the conventional seed propagation method is slow. Tissue culture ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 闫海霞卜朝阳卢家仕黄昌艳何荆洲王晓国
Owner FLOWER RES INST GUANGXI ACADEMY OF AGRI SCI
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