Electric FISH (fluorescence in situ hybridization) method

Abstract

The invention belongs to the field of biologic information science and particularly relates to an electric FISH (fluorescence in situ hybridization) method. The method comprises the following steps of performing sequence alignment by taking a probe sequence as a search sequence and a target sequence as a database sequence; modifying comparison results by using Perl script blast_parser.pl; deleting the results which are relatively short in segments and relatively poor in consistency from the comparison results; making a scatter plot diagram by taking positions of the probe sequence and the search sequence on the target sequence as ordinates and taking comparison times as abscissas. The method is simple and convenient in operation, the results are reliable and precise due to the fact that the results are verified by real FISH, the time and the cost are reduced, and the resolution is high and the pertinency is high due to the fact that a target DNA (deoxyribonucleic acid) can be a genome, a single chromosome, a specific sequence segment and the like.

Description

technical field [0001] The invention belongs to the field of bioinformatics, and in particular relates to an electronic FISH method. Background technique [0002] Fluorescence in situ hybridization technology was born in the late 1960s. Its principle is to use the principle of complementary base pairing to physically locate probe DNA to target DNA such as chromosomes, interphase nuclei, and DNA fibers. At present, fluorescence in situ hybridization technology is widely used in the physical location of genes, chromosome identification, physical map construction, kinship research and transgenic detection, etc., and has an irreplaceable position in the field of modern molecular cytogenetics. [0003] However, fluorescence in situ hybridization experiments often have high requirements on the experimental skills of the experimenters. A slight error in the experimental process will lead to the failure of the experiment, the result is not ideal, and the experiment needs to consume ...

AI Technical Summary

Problems solved by technology

[0003] However, fluorescence in situ hybridization experiments often have high requirements on the experimental skills of the experimenters. A slight error in the experimental process will lead to the failure of the experiment, the result is not ideal, and the experiment needs to consume a lot of experimental materials.

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