Preparation of bordetella bronchiseptica selective medium

A technology of Bordetella sanguinis and culture medium, applied in the direction of bacteria, etc., can solve the problems of poor separation effect, slow growth of Bordetella bronchiseptica, difficult to exclude growing bacteria, etc., and achieves a high separation rate and shows technological progress. Effect

Inactive Publication Date: 2015-02-11
TECON BIOLOGY CO LTD
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Problems solved by technology

When collecting nasal swabs from pigs to isolate Bordetella bronchiseptica, due to the slow growth of Bordetella bronchiseptica, it i

Method used

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Embodiment

[0014] 1) Medium preparation: Take 20g NaCl, 20g peptone, 18g agar powder, 10g glucose, 10g lactose, 1.5g No. 3 bile salt, 3ml 1% neutral red aqueous solution, 100ml calf serum, add distilled water to 1000ml, fully After dissolving and mixing, sterilize under high pressure at 115°C for 15 minutes, cool the high-pressure medium to 55°C, add 500ul of 1% furazolidone dimethylformamide solution, 100mg of ampicillin, 50mg of cyclohexanone, and 10mg of spectinomycin, Mix well and pour into plates, each plate has 20ml (diameter of plate is 90mm).

[0015] 2) Medium preparation: Take 20g NaCl, 20g peptone, 18g agar powder, 10g glucose, 10g lactose, 1.5g No. 3 bile salt, 3ml 1% neutral red aqueous solution, 100ml calf serum, add distilled water to 1000ml, add Mix 550 ul of 1% furazolidone dimethylformamide solution, 110 mg of ampicillin, 55 mg of cyclinone, and 11 mg of spectinomycin, mix well and pour into a plate, each plate has 20 ml (diameter of the plate is 90 mm).

[0016] 3) Me...

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Abstract

The invention relates to preparation of a bordetella bronchiseptica selective medium. The preparation comprises the following steps: taking 120g of NaCl, 20g of peptones, 18g of agar powder, 10g of glucose, 10g of lactose, 1.5g of No.3 cholate, 3ml of a 1% neutral red aqueous solution and 100ml of neonatal bovine serum; adding distilled water to a fixed volume of 1000ml; after dissolving and uniformly mixing, autoclaving for 15 minutes at 115 DEG C; cooling to 55 DEG C; adding a proper amount of a 1% furazolidone dimethylformamide solution, ampicillin, actidione and spectinomycin, taking 20ml of the solution, uniformly mixing and pouring into a plate with the diameter of 90mm; drying, collecting cotton swabs of naval cavities of pigs with atrophic rhinitis clinical symptoms; placing the cotton swabs into 2ml of a sterile PBS (phosphate buffer solution) solution; after fully and uniformly mixing, coating 50 microlitres on the selective medium; culturing for 48 hours at 37 DEG C; and picking up suspicious colonies and identifying, wherein the separation rate of the selective medium is remarkably higher than that of existing separating mediums.

Description

technical field [0001] The invention relates to the preparation of a biological culture medium, and is especially suitable for the isolation and identification of Bordetella bronchiseptica. The selection of the selective culture medium can eliminate the interference of miscellaneous bacteria and improve the separation effect. Background technique [0002] Porcine atrophic rhinitis can cause turbinate atrophy in pigs, destroy the immune barrier of the nasal mucosa, and then cause other respiratory diseases, resulting in significant economic losses for the pig industry. Studies have shown that Bordetella bronchiseptica can cause non-progressive atrophic rhinitis, but infection of pigs with Bordetella bronchiseptica can promote the colonization of Pasteurella multocida, which can lead to severe irreversible turbinate atrophy. When collecting nasal swabs from pigs to isolate Bordetella bronchiseptica, due to the slow growth of Bordetella bronchiseptica, it is difficult to exclud...

Claims

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Application Information

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IPC IPC(8): C12N1/20
CPCC12N1/20
Inventor 何传雨郝成武何海贺笋
Owner TECON BIOLOGY CO LTD
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