A Method for Correcting Particle Imaging Defects in Flow Cells

A particle imaging and flow cell technology, applied in image enhancement, image data processing, instruments, etc., can solve problems such as uneven background illumination and optical system speckle effects, achieve clear images, reduce speckle effects, and be easy to implement. Effect

Active Publication Date: 2017-03-01
DIRUI MEDICAL TECH CO LTD
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  • Description
  • Claims
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Problems solved by technology

[0008] The invention provides a method for correcting particle imaging defects in a flow cell to solve the problems of uneven background illumination and the influence of optical system spots in the microscopic imaging system used in medical in vitro diagnosis

Method used

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  • A Method for Correcting Particle Imaging Defects in Flow Cells
  • A Method for Correcting Particle Imaging Defects in Flow Cells
  • A Method for Correcting Particle Imaging Defects in Flow Cells

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Embodiment Construction

[0030] The applicable structure of the present invention is that the lighting part is a light source that is fixed relative to the flow cell after assembly and adjustment, and cannot be moved relative to each other during the imaging process. The distance is 150-300mm, and its brightness can be adjusted under program control. The deviation is less than 5 degrees; the camera is fixed relative to the flow cell after being adjusted to the focus position, and cannot be moved relative to the imaging process; the camera is a single camera and cannot be switched. The shooting target is the flowing liquid containing particles in the flow cell (transparent container), and the structure is shown as figure 1 As shown, a relatively fixed optical imaging system is very important, so that the system parameters do not change, and the pre-extracted system information can still be used to correct the images taken in the working state.

[0031] (1) Take m groups of pure transparent liquid pictu...

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Abstract

The invention relates to a method for correcting imaging defects of particles in a flow cell and belongs to a background correction method for a flow cell particle imaging system. Pure liquid is adopted to flow in the flow cell in advance; a plurality of groups of images are shot under different brightnesses and are used as estimation data for the state of an integral system; aiming at each pixel, an uneven lighting degree is subjected to statistical fitting; a correct fitting curve of each pixel is stored; when a target object is shot, fitting data is used for regulating the gray level of each pixel of the shot image to a corresponding gray level grade so as to complete gray level correction and fulfill the aims of enabling the image to be clear and enabling the background to be consistent. The method has the advantage of providing a technical means which is simply designed, has a high computing speed and can measure the state of the integral system so as to correct the shot image.

Description

technical field [0001] The invention relates to a method for correcting the background of a particle imaging system in a flow cell, in particular to a correction technology for the influence of uneven background illumination of a microscopic imaging system used in medical in vitro diagnosis and optical system spots. Background technique [0002] The consistency of the background plays a vital role in image analysis. If the background grayscale field is severely distorted, the captured object will also become distorted in grayscale. In severe cases, it may even be unrecognizable, let alone further analysis and processing. Therefore, it is very meaningful to correct the background gray field of imaging. [0003] For the use of transmitted light to shoot particles in the fluid in a transparent container (hereinafter referred to as the flow cell), in a non-completely closed fixed shooting device, it is inevitable that the lens and the surface of the flow cell will be stained, so...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G06T5/50
Inventor 张元囝王强
Owner DIRUI MEDICAL TECH CO LTD
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