Primers and method for quickly detecting Takifugu obscurus young fish sex difference single base mutation

A technique of puffer obscurus and single base mutation, applied in biochemical equipment and methods, microbe measurement/testing, DNA/RNA fragments, etc., can solve the problems of lack of basic research on all-male seed production

Active Publication Date: 2015-02-25
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
View PDF4 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, the research on the cultivation of all-male puffer puffers obscura is limited to the period of sex differentiation and the expression of sex differentiation-related genes (Li Yanyan, Dai Qi, Guo Zhenglong, Zhu Yongxiang, Huang Bo, Zhou Zhongliang, 2011. Dark pattern Pufferfish ( Takifugu obscurus ) Sex differentiation histology and aromatase gene expression. Fudan Journal (Natural Science Edition), 645-652.) and the effect of aromatase inhibitors on sex differentiation and related aromatase gene expression (Huang Bo, Yang Xiaoyu, Dai Qi, Wang Qi, Yun Dan, Zhou Zhongliang, 2013. Effects of aromatase inhibito...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primers and method for quickly detecting Takifugu obscurus young fish sex difference single base mutation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The method for rapidly detecting the gender difference of puffer puffer juveniles described in the present invention comprises the following steps:

[0029] 1. Sample collection

[0030] In this example, puffer puffer juveniles of different sexes were used as the research objects, and all 30 individuals were randomly selected from the juvenile puffer puffer fish population cultured by Jiangsu Zhongyang Group. The gonad tissues of each individual were fixed in Bouin’s solution for 24 hours, rinsed with 50% ethanol and stored in 70% ethanol; the fin ray tissues were cut and stored at -20°C.

[0031] 2. Gonad tissue section and sex differentiation identification of puffer puffer obscura juveniles

[0032] The fixed gonadal tissue samples of juvenile puffer puffer fish were sliced ​​by conventional methods, with a thickness of 4-7 μm, and stained with H.E. Observed and photographed with OLYMPUS DP72 microscope, among the 30 juvenile puffer pufferfish selected, the ratio o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses primers and a method for quickly detecting Takifugu obscurus young fish sex difference single base mutation. According to the mononucleotide mutant sites on sex difference genes of Takifugu obscurus male and female individuals, a pair of external PCR (polymerase chain reaction) primer and internal PCR primer containing mispaired base for the mutant sites are designed to respectively amplify the sex difference gene sequences. By utilizing the primers, different sexes of DNA (deoxyribonucleic acid) samples of Takifugu obscurus can obtain different amplification results, thereby judging the sex difference of Takifugu obscurus. The method has the advantages of high speed and high accuracy, is simple to operate and suitable for popularization and application, can quickly and accurately identify the sex of the Takifugu obscurus young fish on the basis of extracting genome DNA (deoxyribonucleic acid) of Takifugu obscurus under 1 year, and has important application values in accelerating the Takifugu obscurus unisexual breeding progress and researching the quick PCR detection method in the field of molecular biology.

Description

technical field [0001] The invention relates to a primer and a method for rapidly detecting the single base mutation of the sex difference of fugu obscura juveniles. Background technique [0002] Fugu obscurus ( Takifugu Obsecurus ), commonly known as puffer fish, belongs to Osteichthyes, Pufferidae, Pufferidae, and Fugu genus. It is mainly distributed in the northern coastal areas of my country's coastal waters (East China Sea, Yellow Sea, Bohai Sea) and the middle and lower reaches of the Yangtze River. It has the habit of anadromous migration. Fattening and maturing in the sea, broodstock go up the river from the sea to spawn in fresh water in spring, larvae grow in the Yangtze River and lakes, and juveniles enter the sea in the spring of the following year. Fugu obscurus itself does not produce toxins, but it can accumulate toxins in prey organisms, especially in ovary, liver, blood and skin, etc., and eat wild puffer obscurus without special treatment and cooking by mis...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6858C12Q1/6879C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 刘滨胡鹏刘新富孟振杨志
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap