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Preparation method of nanobiosensor for 2,4,6-TNT detection

A 6-TNT, bio-nano technology, used in biological testing, material inspection products, color/spectral property measurement, etc., can solve the problems of high animal training cost, insufficient sensor sensitivity and specificity, and achieve biological structure stability. Good, high sensitivity, low cost effect

Active Publication Date: 2015-03-11
ZHEJIANG UNIV
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Problems solved by technology

However, the cost of animal training is very high, and it is very uncontrollable
However, the complete electronic detection device has a certain gap between its physical or chemical sensing function and the biochemical sensing process that occurs in the biological olfactory system, so that the sensor is still insufficient in terms of sensitivity and specificity.

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  • Preparation method of nanobiosensor for 2,4,6-TNT detection
  • Preparation method of nanobiosensor for 2,4,6-TNT detection
  • Preparation method of nanobiosensor for 2,4,6-TNT detection

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Embodiment Construction

[0016] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, but the present invention is not limited.

[0017] The present invention is used for the preparation method of bionano sensor of 2,4,6-trinitrotoluene detection, comprises the following steps:

[0018] 1. Synthesis of 2,4,6-trinitrotoluene-specific and sensitive peptide sequence: use standard Fmoc solid-phase synthesis method from carboxy-terminal (C-terminal) to amino-terminal (N-terminal) to repeat deprotection and activation according to the peptide sequence WHWQRPLMPVSID 3. Add amino acids in a 3-step coupling cycle, purify and extract as 100 μg / ml polypeptide solution and save (solvent is 0.1M PBS buffer, pH=7.2, 0.1M refers to the molar concentration of phosphate in PBS buffer, used in the present invention All the PBS buffer refers to 0.1 M PBS buffer with pH=7.2).

[0019] 2. Thiolation modification of specific and sensitive polypeptid...

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Abstract

The invention discloses a preparation method of a nanobiosensor for 2,4,6-TNT detection. According to the method, 2,4,6-trinitrotoluene (2,4,6-TNT) specific sensitive sulfhydrylized polypeptide (WHWQRPLMPVSID-SH) is synthesized by a standard chemical synthesis method and is fixed to the surface of a nanosensor by covalent linkage between sulfydryl and nanometal particles. During detection, specific binding between the target molecule 2,4,6-trinitrotoluene and sulfhydrylized polypeptide leads to changes of optical refractive property of the nanosensor surface. Thus, corresponding spectrum changes of transmission light penetrated by the sensor are caused, and detection of the target molecule 2,4,6-trinitrotoluene is realized. In comparison with a biosensor for detection of 2,4,6-trinitrotoluene, the nanobiosensor provided by the invention has advantages of high sensitivity, good biological structure stability and low cost due to sulfhydrylized polypeptide modification.

Description

technical field [0001] The invention relates to a preparation technology of a biosensor, in particular to a preparation method of a bionano sensor used for detecting explosive 2,4,6-trinitrotoluene (2,4,6-TNT). Background technique [0002] The rapid and sensitive detection technology of explosive 2,4,6-trinitrotoluene has a very important demand in the field of public safety. For its on-site detection, generally, trained animals such as police dogs are used for inspection, or rapid detection instruments based on traditional physical and chemical methods are used. However, the cost of animal training is very high, and it is highly uncontrollable. However, there is a certain gap between the physical or chemical sensing function of a complete electronic detection device and the biochemical sensing process that occurs in the biological olfactory system, so that the sensor is still insufficient in terms of sensitivity and specificity. Peptide is a small protein molecule that c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/25
CPCG01N21/25G01N33/68
Inventor 刘清君张迪鸣卢妍利张倩姚瑶李爽
Owner ZHEJIANG UNIV