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Sparassis crispa liquid strain culture medium and culture method

A technology of liquid strains and culture methods, which is applied in the direction of botany equipment and methods, fungi, fertilizer mixtures, etc., can solve the problems of high cost of hydrangea liquid culture medium and limited source of raw materials, and achieve convenient source of raw materials and easy operation Convenience, the effect of simple cultivation process

Inactive Publication Date: 2015-03-25
INST OF EDIBLE FUNGI FUJIAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the disadvantages of high cost of hydrangea liquid culture medium and limited sources of raw materials, the present invention provides a hydrangea liquid strain culture medium and a cultivation method, which reduces production costs and improves production efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Hydrangea liquid stock culture

[0033] (1) Mother species activation. Inoculate hydrangea in PDA (peeled potatoes 200g, glucose 20g, agar powder 16g) slant medium, and culture at 23-25°C for 25 days to obtain activated hydrangea parent species;

[0034] (2) Prepare the liquid stock. Peeled potatoes 200g sliced, add 1L water to boil, filter and add glucose 20g, peptone 2g, KH 2 PO 4 3g, MgSO 4 ·7H 2 O 1g, after stirring evenly, set the volume to 1L, and adjust the pH to 5.5;

[0035] (3) Divide the above-mentioned liquid culture medium into 250ml Erlenmeyer flasks, with a liquid volume of 100ml, and sterilize with high-pressure steam for 30 minutes;

[0036] (4) After sterilization, inoculate 5-8 pieces of activated slant mother species (0.5cm × 0.5cm), culture in a shaker with a rotation speed of 150rpm and a temperature of 23-25°C. After 10 days of cultivation, the liquid mother species will be obtained.

[0037] 2. Hydrangea liquid stock culture

[0038] ...

Embodiment 2

[0047] 1. Hydrangea liquid stock culture

[0048] Mother species activation and liquid mother species cultivation were carried out according to the method in Example 1.

[0049] 2. Hydrangea liquid stock culture

[0050] (1) Liquid stock medium: glutinous rice flour 70g, fish meal peptone 1g, KH 2 PO 4 1.5g, MgSO 4 ·7H 2 O 3g, the initial pH is 5.

[0051] Control medium: 200g of potatoes, 20g of glucose, 2g of fishmeal peptone, the pH value is natural, and the volume is adjusted to 1L.

[0052] (2) Inoculate the cultured liquid stock culture medium into the above-mentioned liquid stock culture medium, the inoculum amount is 8%, the culture temperature is 23-25°C, the rotation speed of the shaker flask is 150rpm, and the shaking culture is carried out under natural light conditions for 15 days. Get hydrangea liquid stock. According to the method in Example 1, the diameter of the bacterium ball is 1.9 mm, the density of the bacterium ball is 49.3 / ml, and the mycelial bi...

Embodiment 3

[0055] 1. Hydrangea liquid stock culture

[0056] Mother species activation and liquid mother species cultivation were carried out according to the method in Example 1.

[0057] 2. Hydrangea liquid stock culture

[0058] (1) Liquid stock culture medium: glucose 10g, glutinous rice flour 60g, fish meal peptone 2g, KH 2 PO 4 3g, MgSO 4 ·7H 2 O 1.5g, initial pH was 5.5.

[0059] Control medium: 200g of potatoes, 20g of glucose, 2g of fishmeal peptone, the pH value is natural, and the volume is adjusted to 1L.

[0060] (2) Inoculate the cultured liquid stock culture medium into the above-mentioned liquid stock culture medium, the inoculum amount is 8%, the culture temperature is 23-25°C, the rotation speed of the shaker flask is 150rpm, and the shaking culture is carried out under natural light conditions for 15 days. Get hydrangea liquid stock. According to the method in Example 1, after determination, the diameter of the bacterium ball is 1.7 mm, the density of the bacte...

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Abstract

The invention belongs to the technical field of industrial cultivation for edible funguses, and particularly relates to a sparassis crispa liquid strain culture medium and a culture method. The formula of the culture medium comprises 0-145 g / L of glucose, 0-70 g / L of glutinous rice flour, 1-6 g / L of fish meal peptone, 0-5 g / L of KH2PO4 and 0-3 g / L of MgSO4.7H2O, the original pH is 5-6, and the culture medium is cultured by preparation of sparassis crispa liquid mother strains and preparation of sparassis crispa liquid original strains. Aiming at the defects that the sparassis crispa liquid culture medium is high in cost and limited in raw material sources, the invention provides the sparassis crispa liquid strain culture medium and the culture method. The production cost is reduced, and the production efficiency is improved. The liquid culture medium has the advantages of being simple in formula and convenient in raw material sources and is suitable for large-scale production. The liquid strain culture process is simple, and the operation is convenient.

Description

technical field [0001] The invention belongs to the technical field of industrial cultivation of edible fungi, and in particular relates to a hydrangea liquid culture medium and a cultivation method. Background technique [0002] Hydrangea ( Sparasis latifolia ), also known as hydrangea mushroom and hydrangea mushroom, are named for their zigzagging and huge hydrangea-shaped seeds. They are known as dreamy and magical mushrooms in Japan. It is as rare as Cordyceps sinensis, morels, truffles and other treasures. According to the measurement, the content of crude protein and crude fiber is 12.9% and 13.7% in the dry fruiting body of Hydrangea hydrangea. (30.6 mg / kg) and so on are relatively high, and the vitamin content is also very rich, such as vitamin C, vitamin D, vitamin E, etc., among which the content of vitamin E ranks in the forefront of bacteria and algae foods in the "Food Composition Table". Hydrangea is not only delicious, high nutritional value, but also has g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05G1/00
CPCC12N1/14
Inventor 马璐林衍铨江晓凌应正河
Owner INST OF EDIBLE FUNGI FUJIAN ACAD OF AGRI SCI
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