Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for creating a novel restorer line of special-shaped leaves of Brassica napus

A cabbage type rape and restorer technology is applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of high seed production risk, difficult to guarantee purity and high cost, and achieves reduction of seed production cost and seed production. Risk, the effect of improving the purity of seed production

Inactive Publication Date: 2016-08-17
贵州省油菜研究所 +1
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a special-shaped Brassica napus in view of the problems in the prior art such as low yield, high cost, strict isolation conditions to the surrounding environment, difficulty in ensuring purity, and high risk of seed production. A method created by a new leaf restorer line, which has high seed production yield, low cost, no need for isolation, easy guarantee of seed purity, and low risk of seed production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for creating a novel restorer line of special-shaped leaves of Brassica napus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Select the time around 10:00 in the morning, and use tweezers to pick the flower buds of the Brassica napus restorer line W-1009 (high oil content yellow seed double low line) in the field for microspore culture, and add 0.02-0.2 % concentration of colchicine was induced to double, and 55 mutant plants were obtained through culture, and 2 mutants with specific leaf morphology were selected (1-6 leaflets of different sizes were grown on both sides of the veins of normal leaves, such as attached figure 1 As shown in the right b leaf in the middle, a small leaf grows on both sides of the vein), which was transplanted to the field after tissue culture and seedling hardening.

[0022] 2. When transplanted to the field, all mutant plants are bagged and self-bred, F 1 Segregation of mutant traits occurs, in F 1 In the population, 5 normal individuals and 10 mutant individuals were selected for self-bagging, and it was found that all the offspring of the normal individuals ...

Embodiment 2

[0030] 1. Select the time around 10 am in the field to pick the flower buds of the Brassica napus restorer line W-1156 (high oil content black seed double low line) with tweezers in the field for microspore culture, and add 0.02-0.2% during the doubling culture period The concentration of colchicine was induced to double, and 69 mutant plants were obtained through culture. Two mutants with specific leaf morphology were selected (1-6 leaflets of different sizes were grown on both sides of the veins of normal leaves). Transplant to field after seedlings.

[0031] 2. When transplanted to the field, all mutant plants are bagged and self-bred, F 1 Mutant traits segregated, and 5 normal individuals and 10 mutant individuals were selected in the F1 population to carry out self-bagging, and it was found that all the offspring of the normal individuals were normal; while 4 of the 10 mutant individuals No trait segregation occurred in the progeny, and all the leaves of a single plant s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for creating a novel recovery system of brassica napus type rape heterogenic leaves. The method comprises the following steps: selecting excellent brassica napus type rape restorer line for performing microspore culture, performing inducing and doubling treatment by 0.02-0.2% colchicine during a culture period to obtain a heterogenic leaf mutant strain; self-crossing the mutant strain by two generations, selecting an individual plant from a segregation population without mutant characters; then, taking the original excellent brassica napus type rape restorer liner as a recurrent parent for performing backcrossing and self-crossing, and selectively breeding the novel heterogenic leaf restorer line from the next-generation segregation population to form a novel strong superiority hybrid combined rape variety with a sterile line. The method disclosed by the invention can be used for effectively improving the seed production purity and the seed production yield, lowering the seed production and the seed production risk, and is free of need of isolation. The excellent restorer line is used at the initial stage for performing genetic improvement on the mutant strain, and a sterile line blood relationship is not introduced, and therefore, the method is beneficial for performing population division on the sterile line restorer line, and easily screening and preparing the strong superiority hybridized combination.

Description

technical field [0001] The invention relates to the field of crop genetics and breeding, specifically to the field of rapeseed crop breeding, in particular to a method for creating a novel restorer line of Brassica napus with special-shaped leaves. Background technique [0002] my country is a big consumer of edible vegetable oil, and the supply of domestic edible vegetable oil has been insufficient for a long time. At present, more than 60% of domestic edible vegetable oil is imported; rapeseed is the main source of edible vegetable oil, accounting for more than 50% of the total edible vegetable oil. In 2012, my country's imported rapeseed reached 2.93 million tons. By 2015, my country's rapeseed import volume will exceed 3 million tons. If the country cancels the restrictive policy, the rapeseed import volume may reach or even exceed 4 million tons. The hidden danger of rapeseed supply security is becoming increasingly prominent. Therefore, it is of great significance to in...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/06
Inventor 伍林涛奉斌杜才富韩宏仕秦信蓉曾章丽
Owner 贵州省油菜研究所