Tissue culture method of cut flower chrysanthemum Jianyehuang
A tissue culture, cut chrysanthemum technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the difficulties of callus induction and rooting induction, hinder variety standardization and industrial production, and the proportion of domestic cut chrysanthemum varieties. Small share and other problems, to achieve the effect of improving product quality, improving production status, and meeting product demand
Inactive Publication Date: 2015-03-25
HENAN AGRICULTURAL UNIVERSITY
View PDF3 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Due to the difficulty of callus induction and rooting induction in the tissue culture process, it seriously hinders the standardization and factory production of this variety, and the share of domestic cut chrysanthemum varieties is relatively small
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0019] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.
[0020] Such as figure 1 Shown, the present invention comprises the steps:
[0021] Selection of explants: Select the young leaves or stems of cut chrysanthemums with yellow leaves or stems, which are robust and free of diseases and insect pests.
[0022] Disinfection: After rinsing the explants with streamlined tap water for 2 hours, put them in a beaker and soak them in sterile water for 2 hours; then immerse the material in 75% alcohol on a sterile ultra-clean workbench for 10 seconds and rinse with sterile water 4 times , and then transferred to 0.1% mercuric chloride solution for sterilization, leaves for 4 minutes and stems for 3 minutes; then washed with sterile water for 5 times, blotted dry with sterile filter paper, and then inoculated.
[0023] Cultivation: the cultivation phase includes leaf tissue culture and stem segment tissue culture; s...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses a tissue culture method of cut flower chrysanthemum Jianyehuang. The tissue culture method comprises the following steps: selecting an explant, namely selecting a cut flower chrysanthemum Jianyehuang tender leaf or stem which has a completely-expanded terminal bud, grows robustly and has no plant diseases and insect pests; disinfecting; culturing, wherein a culturing manner comprises leaf tissue culture and stem tissue culture; inducing the bud, wherein a bud induction phase is divided into two time periods: the temperature of the first period is 25 DEG C, the light illumination is 60% and the duration is 10 hours, the temperature of the second period is 24 DEG C, the light illumination is 0% and the duration is 10 hours; carrying out subculture multiplication, namely after carrying out subculture on the induced bud, inoculating the bud into a culture medium, wherein the culture medium is composed of 2mg / L of MS+6-BA and 0.3mg / L of NAA; and inducing and rooting, namely obtaining a seedling. According to the tissue culture method, the leaf and stem tissues of the cut flower chrysanthemum Jianyehuang are used as the explant to be subjected to regeneration culture, and a tissue culture rapid reproduction system is established, and the product requirements of a cut flower chrysanthemum market are met.
Description
technical field [0001] The invention relates to a tissue culture method of cut chrysanthemum, in particular to a tissue culture method of cut chrysanthemum chrysanthemum. Background technique [0002] Chrysanthemum is the perennial root herbaceous plant of Compositae Chrysanthemum, is one of four large cut flowers in the world. Traditionally, chrysanthemum is mainly propagated by cuttings and divisions, but these two methods are easily restricted by seasons and external environmental conditions, and the reproduction coefficient is low and the quality of seedlings is poor. With the increasing quality requirements of the flower market, more and more enterprises have begun to standardize and factory-produce chrysanthemum seedlings, and tissue culture is the key technology in this production process. The cut chrysanthemum variety 'Jianyehuang' is an important domestic cut flower variety, with yellow flowers, 4-7 cm flower diameter, flowering period from August to November, and ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More IPC IPC(8): A01H4/00
Inventor 李永华张开明李永贺丹张凌王政雷雅凯张曼张淑梅蒋鹤
Owner HENAN AGRICULTURAL UNIVERSITY