Application of wheat AX (arabinoxylan) extracts to promoting DNA (deoxyribonucleic acid) transcription of intestinal tracts

Abstract

The invention discloses an application of wheat AX (arabinoxylan) extracts to promoting DNA (deoxyribonucleic acid) transcription of intestinal tracts. The wheat AX extracts are prepared by a method comprising the following steps: carrying out centrifugal separation on industrial wastewater generated after extracting wheat gluten and / or wheat starch from wheat flour, and taking a supernatant; cooking the supernatant, and then carrying out centrifugal separation on the supernatant to obtain a supernatant; obtaining the wheat AX extracts by 1) carrying out spray drying on the supernatant, or 2) concentrating the supernatant, regulating the pH value to be 7-8, then adding beta-amylase and glucoamylase to react, finally obtaining a supernatant after centrifugal separation and precipitating the supernatant with ethanol. The wheat AX extracts also have the functions of improving the intestinal tract barrier functions, promoting growth of small intestinal villi, reducing the pH values of the intestinal tracts and increasing the contents of short-chain fatty acids in the intestinal tracts.

Description

technical field [0001] The invention relates to an application of wheat arabinoxylan extract in promoting intestinal DNA transcription. Background technique [0002] Arabinoxylans (AX for short) is a hemicellulose that exists in the primary and secondary cell walls of plants, including grain seeds and wood. AX is a polymer composed of two sugars, xylose and arabinose, also known as pentosan. The basic structure includes the main chain of xylan and the side chain of arabinose, which are connected by xylose residues through 1,4-glycosidic bonds, and there are also phenolic acids connected by ester bonds on some arabinose. [0003] At present, there are two main sources of wheat arabinoxylan extraction, one is extracted from wheat bran, and the other is extracted from wheat flour. The extraction methods mainly include alkaline extraction and enzyme extraction. [0004] Bran is a by-product of wheat processing, and the content of arabinoxylan is rich, about 20% or more. The an...

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Problems solved by technology

[0004] Bran is a by-product of wheat processing, and the content of arabinoxylan is rich, about 20% or more. The annual output of bran in my country is as high as 20 million tons. However, most of the arabinoxylans in the bran are insoluble in water and difficult to extract. Currently, the feasible method is alkaline extraction. The alkaline extraction process will bring a large amount of alkali-containing wastewater to be discharged, causing environmental pollution

[0005] The content of arabinoxylan in flour is about 2%, of which the water-extractable part accounts for about 0.5% to 0.8%. Extracting arabinoxylan directly from flour requires a large amount of flour raw materials and high extraction costs. Currently, it is only in the experiment Small-scale extraction is carried out in the laboratory, and the extraction method is mostly physical centrifugation combined with enzymatic method, which is only used for scientific research, and the exploration of large-scale industrial production is not enough. There is no mature process route, and industrial production cannot be carried out.

[0006] Wheat starch and gluten production wastewater contains high-quality water-soluble arabinoxylan. The wheat flour processing industry in my country is huge. The traditional wheat starch and gluten processing technology is to obtain wheat flour through water extraction and separation. For the target substance, the elution water is discarded. For every ton of starch produced, the discharge of high-concentration organic wastewater is between 5 and 12 tons. The direct discharge of these organic wastewater will cause serious pollution to the environment.

At present, there is no domestic enterprise that has effectively utilized these processing waste water. The analysis of the reasons is that, on the one hand, starch and grain powder enterprises have not realized the utilization value of the eluted waste water, and the physiological activity and processing characteristics of arabinoxylan have not been recognized. The application value is not understood. On the other hand, there is currently no efficient and mature separation and extraction process, and it is impossible to utilize a large amount of eluted wastewater

[0007] Domestic studies on the physiological activity of arabinoxylan mostly focus on the arabinoxylan derived from wheat bran, but there are few reports on the physiological activity of arabinoxylan extracted from wheat flour

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