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Radish EST-SSR core primer combination and application thereof

A core primer and primer combination technology, applied in the biological field, can solve problems such as difficult sequence comparison, and achieve the effects of good repeatability, high polymorphism, and stable and reliable labeling

Active Publication Date: 2015-03-25
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Theoretically, only the comparison of the whole genome sequence of each variety can correctly reflect the specificity of different varieties. However, most plant genome sequencing projects have not been completed at present, and it is difficult to perform sequence comparison. Only by repeatedly screening core primer combinations can an ideal test be obtained. result

Method used

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  • Radish EST-SSR core primer combination and application thereof
  • Radish EST-SSR core primer combination and application thereof
  • Radish EST-SSR core primer combination and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Example 1 Study on Genetic Diversity of Radish Breeding Materials

[0029] 1. Materials and methods:

[0030] 1.1 Materials

[0031] The test materials were 93 germplasm resources of radish.

[0032] The 20 radish materials used for primer screening cover 6 subspecies of Radish cultivars R.sativus var.sativus L. (cherry radish), var.hortensis Becker (East Asian long radish), var.niger Kerner (black radish ), var.oleiferus Metzg (oil radish), var.caudatus Hooker and Anderson (long pod radish) and R.sativus var.raphanistroides Makino (East Asian wild radish); 3 wild radish species R.raphanistrum, R.landra and R .maritimus. Rich in types, including different skin colors (red, green, white, black), different root types (round, long cylinder, ellipse, etc.), and different sizes of radish resources, basically covering the main ecological types and main agronomic traits of radish. The germplasm diversity is reflected in many places, with high genetic diversity (Table 1). ...

Embodiment 2

[0056] Embodiment 2 The effectiveness test of 21 pairs of SSR core primer combinations of the present invention

[0057] In order to further test the effectiveness of the 21 pairs of core primers in the identification of radish genetic diversity and genetic relationship, more breeding materials were selected as the analysis objects, which not only increased the number of the same type of materials but also included sister lines and wild For materials with close genetic relationship such as different individual plants in the resources, 21 core primer combinations were used for cluster analysis.

[0058] UPGMA cluster analysis showed that ( Figure 4 ), the genetic similarity coefficients of the 93 tested materials ranged from 0.58 to 0.99. All materials can be divided into 4 major groups (I, II, III and IV) at the genetic similarity coefficient of 0.63. Group I contains 86 materials belonging to cultivated radish type (R. sativus). Group II contains 5 wild Radish material bel...

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Abstract

The invention discloses a radish EST-SSR core primer combination which comprises primers of which nucleotide sequences are as shown in SEQ ID NO. 1-42. The SSR core primer combination disclosed by the invention has the advantages of showing genetic diversity of a radish germplasm material, stable and reliable labeling, good repeatability, high polymorphism, convenience in statistics and the like to the greatest extent; when being used in genetic diversity analysis, the primer combination can be used for reflecting the genetic relationship of a tested material to the greatest extent with the fewest primers.

Description

technical field [0001] The invention relates to a set of radish EST-SSR core primer sequences and applications thereof, belonging to the field of biotechnology. Background technique [0002] Radishes have a long history of cultivation in China, are widely distributed, and have a wide variety of varieties. According to the statistics of the Ministry of Agriculture in recent years, the annual planting area is about 18 million mu, ranking among the top three vegetable planting areas in my country, and occupies an important position in the annual supply of vegetables in China. However, the current Chinese radish seed market is chaotic, and there are serious problems with the authenticity of varieties caused by the same thing with different names or different names with the same name in the market. The quality problems of high-grade seeds are also becoming more and more prominent, which seriously interferes with the healthy development of the radish seed industry. The current p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 张丽王庆彪
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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