A kind of method for cultivating Cordyceps militaris stroma in liquid
A technology of Cordyceps militaris and Cordyceps militaris, which is applied in the field of liquid cultivation of Cordyceps militaris, can solve problems such as pesticide residues, food safety hazards, and lack of production technology and methods, so as to increase the content of active ingredients, easy to control growth conditions, Ease of industrial production
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Embodiment 1
[0040] A method for cultivating Cordyceps militaris subunits in a liquid is to inoculate Cordyceps militaris strains on the culture medium to cultivate; comprising the following steps:
[0041] A. The cultivation of mycelium
[0042] The culture medium is prepared with the following raw materials:
[0043] 220 grams of potatoes; 10 grams of glucose; 0.5 grams of potassium dihydrogen phosphate; 0.5 grams of magnesium sulfate; vitamin B 1 20 milligrams; 1.5 grams of sodium chloride; 3 grams of peptone; 1000 ml of water; granular attachments accounting for 30% of the volume of the fermentation medium; pH=7.5;
[0044] The culture conditions are as follows:
[0045] The temperature was 21°C for 48 hours, and the rotation speed was 100rpm and the condition was 21°C for 4 days;
[0046] B. The cultivation of fruiting bodies
[0047] B1, post-ripening period
[0048] The culture conditions are as follows:
[0049] The ambient humidity is 65%, and the cultivation is carried out in...
Embodiment 2
[0061] The difference between this embodiment and embodiment 1 is:
[0062] A. The cultivation of mycelium
[0063] The culture medium is prepared with the following raw materials:
[0064] Potato 250g; Glucose 20g; Potassium dihydrogen phosphate 1g; Magnesium sulfate 1g; Vitamin B 1 20 mg; 1.8 grams of sodium chloride; 4.5 grams of peptone; 1000 ml of water; granular attachments accounting for 50% of the volume of the fermentation medium; pH=7.5;
[0065] The culture conditions are as follows:
[0066] The temperature is 27°C and the temperature is 72 hours, and the rotation speed is 150rpm, and the temperature is 27°C and cultivated for 6 days;
[0067] B. The cultivation of fruiting bodies
[0068] B1, post-ripening period
[0069] The culture conditions are as follows:
[0070] The ambient humidity is 75%, 20°C for 7 days in the dark, and after the secondary growth of mycelia on the surface of the fermentation broth to completely cover the surface of the medium, move...
Embodiment 3
[0079] The difference between this embodiment and embodiment 1 is:
[0080] A method for cultivating Cordyceps militaris subunits in a liquid is to inoculate Cordyceps militaris strains on the culture medium to cultivate; comprising the following steps:
[0081] A. The cultivation of mycelium
[0082] The culture medium is prepared with the following raw materials:
[0083] 230 grams of potatoes; 150 grams of glucose; 0.8 grams of potassium dihydrogen phosphate; 0.8 grams of magnesium sulfate; vitamin B 1 20 milligrams; 1.7 grams of sodium chloride; 4 grams of peptone; 1000 ml of water; granular attachments accounting for 40% of the volume of the fermentation medium; pH=7.5;
[0084] The culture conditions are as follows:
[0085] The temperature is 24°C and the temperature is 60 hours, and the rotation speed is 130rpm, and the temperature is 24°C for 5 days;
[0086] B. The cultivation of fruiting bodies
[0087] B1, post-ripening period
[0088] The culture conditions ...
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