A kind of method for cultivating Cordyceps militaris stroma in liquid

Abstract

The invention relates to culture of edible-medicinal fungi, and in particular relates to a liquid culture method for cordyceps militaris stroma. The liquid culture method for cordyceps militaris stroma is that cordyceps militaris strains are inoculated into a culture medium to be cultured. The liquid culture method for cordyceps militaris stroma comprises the steps of culturing mycelium, culturing sporocarp, etc. With the adoption of the liquid culture method for cordyceps militaris stroma, the technical problems of long production cycle, high energy consumption, high potential risk and the like in the prior art can be solved; the liquid culture method for cordyceps militaris stroma has the advantages that the production cycle is reduced, the contents of effective ingredients of cordyceps militaris stroma are increased, the growth conditions are easily controlled, and the industrial production can be carried out conveniently.

Description

technical field

[0001] The invention belongs to the cultivation of edible and medicinal fungi, in particular to a method for cultivating Cordyceps militaris in liquid. Background technique

[0002] Cordyceps militaris is a model species of Ascomycetes subphylum, Ergotaceae, Ergotaceae, Cordyceps, also known as Cordyceps militaris, Cordyceps militaris, Cordyceps and so on. Worldwide distribution of natural resources is very small. It is a complex composed of two parts, the sub-seat (that is, the grass part) and the sclerotia (that is, the dead body part of the insect). Traditional Chinese medicine believes that Cordyceps enters the two meridians of the lung and kidney, which can not only nourish the lung yin, but also nourish the kidney yang. The only traditional Chinese medicine that can balance and regulate Yin and Yang at the same time. Modern medicine believes that its unique pharmacological effects are derived from containing cordycepin and cordyceps polysaccharide. ...

Examples

Embodiment 1

[0040] A method for cultivating Cordyceps militaris subunits in a liquid is to inoculate Cordyceps militaris strains on the culture medium to cultivate; comprising the following steps:

[0041] A. The cultivation of mycelium

[0042] The culture medium is prepared with the following raw materials:

[0043] 220 grams of potatoes; 10 grams of glucose; 0.5 grams of potassium dihydrogen phosphate; 0.5 grams of magnesium sulfate; vitamin B 1 20 milligrams; 1.5 grams of sodium chloride; 3 grams of peptone; 1000 ml of water; granular attachments accounting for 30% of the volume of the fermentation medium; pH=7.5;

[0044] The culture conditions are as follows:

[0045] The temperature was 21°C for 48 hours, and the rotation speed was 100rpm and the condition was 21°C for 4 days;

[0046] B. The cultivation of fruiting bodies

[0047] B1, post-ripening period

[0048] The culture conditions are as follows:

[0049] The ambient humidity is 65%, and the cultivation is carried out in...

Embodiment 2

[0061] The difference between this embodiment and embodiment 1 is:

[0062] A. The cultivation of mycelium

[0063] The culture medium is prepared with the following raw materials:

[0064] Potato 250g; Glucose 20g; Potassium dihydrogen phosphate 1g; Magnesium sulfate 1g; Vitamin B 1 20 mg; 1.8 grams of sodium chloride; 4.5 grams of peptone; 1000 ml of water; granular attachments accounting for 50% of the volume of the fermentation medium; pH=7.5;

[0065] The culture conditions are as follows:

[0066] The temperature is 27°C and the temperature is 72 hours, and the rotation speed is 150rpm, and the temperature is 27°C and cultivated for 6 days;

[0067] B. The cultivation of fruiting bodies

[0068] B1, post-ripening period

[0069] The culture conditions are as follows:

[0070] The ambient humidity is 75%, 20°C for 7 days in the dark, and after the secondary growth of mycelia on the surface of the fermentation broth to completely cover the surface of the medium, move...

Embodiment 3

[0079] The difference between this embodiment and embodiment 1 is:

[0080] A method for cultivating Cordyceps militaris subunits in a liquid is to inoculate Cordyceps militaris strains on the culture medium to cultivate; comprising the following steps:

[0081] A. The cultivation of mycelium

[0082] The culture medium is prepared with the following raw materials:

[0083] 230 grams of potatoes; 150 grams of glucose; 0.8 grams of potassium dihydrogen phosphate; 0.8 grams of magnesium sulfate; vitamin B 1 20 milligrams; 1.7 grams of sodium chloride; 4 grams of peptone; 1000 ml of water; granular attachments accounting for 40% of the volume of the fermentation medium; pH=7.5;

[0084] The culture conditions are as follows:

[0085] The temperature is 24°C and the temperature is 60 hours, and the rotation speed is 130rpm, and the temperature is 24°C for 5 days;

[0086] B. The cultivation of fruiting bodies

[0087] B1, post-ripening period

[0088] The culture conditions ...

technical field

[0001] The invention belongs to the cultivation of edible and medicinal fungi, in particular to a method for cultivating Cordyceps militaris in liquid. Background technique

[0002] Cordyceps militaris is a model species of Ascomycetes subphylum, Ergotaceae, Ergotaceae, Cordyceps, also known as Cordyceps militaris, Cordyceps militaris, Cordyceps and so on. Worldwide distribution of natural resources is very small. It is a complex composed of two parts, the sub-seat (that is, the grass part) and the sclerotia (that is, the dead body part of the insect). Traditional Chinese medicine believes that Cordyceps enters the two meridians of the lung and kidney, which can not only nourish the lung yin, but also nourish the kidney yang. The only traditional Chinese medicine that can balance and regulate Yin and Yang at the same time. Modern medicine believes that its unique pharmacological effects are derived from containing cordycepin and cordyceps polysaccharide. ...

Examples

Embodiment 1

[0040] A method for cultivating Cordyceps militaris subunits in a liquid is to inoculate Cordyceps militaris strains on the culture medium to cultivate; comprising the following steps:

[0041] A. The cultivation of mycelium

[0042] The culture medium is prepared with the following raw materials:

[0043] 220 grams of potatoes; 10 grams of glucose; 0.5 grams of potassium dihydrogen phosphate; 0.5 grams of magnesium sulfate; vitamin B 1 20 milligrams; 1.5 grams of sodium chloride; 3 grams of peptone; 1000 ml of water; granular attachments accounting for 30% of the volume of the fermentation medium; pH=7.5;

[0044] The culture conditions are as follows:

[0045] The temperature was 21°C for 48 hours, and the rotation speed was 100rpm and the condition was 21°C for 4 days;

[0046] B. The cultivation of fruiting bodies

[0047] B1, post-ripening period

[0048] The culture conditions are as follows:

[0049] The ambient humidity is 65%, and the cultivation is carried out in...

Embodiment 2

[0061] The difference between this embodiment and embodiment 1 is:

[0062] A. The cultivation of mycelium

[0063] The culture medium is prepared with the following raw materials:

[0064] Potato 250g; Glucose 20g; Potassium dihydrogen phosphate 1g; Magnesium sulfate 1g; Vitamin B 1 20 mg; 1.8 grams of sodium chloride; 4.5 grams of peptone; 1000 ml of water; granular attachments accounting for 50% of the volume of the fermentation medium; pH=7.5;

[0065] The culture conditions are as follows:

[0066] The temperature is 27°C and the temperature is 72 hours, and the rotation speed is 150rpm, and the temperature is 27°C and cultivated for 6 days;

[0067] B. The cultivation of fruiting bodies

[0068] B1, post-ripening period

[0069] The culture conditions are as follows:

[0070] The ambient humidity is 75%, 20°C for 7 days in the dark, and after the secondary growth of mycelia on the surface of the fermentation broth to completely cover the surface of the medium, move...

Embodiment 3

[0079] The difference between this embodiment and embodiment 1 is:

[0080] A method for cultivating Cordyceps militaris subunits in a liquid is to inoculate Cordyceps militaris strains on the culture medium to cultivate; comprising the following steps:

[0081] A. The cultivation of mycelium

[0082] The culture medium is prepared with the following raw materials:

[0083] 230 grams of potatoes; 150 grams of glucose; 0.8 grams of potassium dihydrogen phosphate; 0.8 grams of magnesium sulfate; vitamin B 1 20 milligrams; 1.7 grams of sodium chloride; 4 grams of peptone; 1000 ml of water; granular attachments accounting for 40% of the volume of the fermentation medium; pH=7.5;

[0084] The culture conditions are as follows:

[0085] The temperature is 24°C and the temperature is 60 hours, and the rotation speed is 130rpm, and the temperature is 24°C for 5 days;

[0086] B. The cultivation of fruiting bodies

[0087] B1, post-ripening period

[0088] The culture conditions ...