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Method for rapidly reproduction overwintering bud of epimedium sagittatum

A rapid technology of Epimedium arrowleaf, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of low induction rate, few applications, difficult rooting, etc., and achieves high reproduction rate, simple operation, The effect of low production cost

Inactive Publication Date: 2015-04-01
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, tissue culture technology is not widely used in the production of Epimedium sagittarius, and there are few reports on the tissue culture technology of Epimedium sagittarius. The existing tissue culture technology of Epimedium sagittarius has low induction rate and difficulty in rooting, etc. problem

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0020] A method for rapid propagation of overwintering buds of Epimedium sagittatum, the steps of which are as follows:

[0021] 1) Disinfection of explants: take the tiller buds formed in the fall of Epimedium that year, that is, the overwintering buds. Scrub the surface of the overwintering buds clean, then rinse them under running water for 30 minutes, 60 minutes or 90 minutes, and dry them until there is no water stain on the surface of the buds. Under aseptic conditions, put the dried overwintering buds in a sterilized jar. Soak in 75% (volume ratio) alcohol solution for 40 seconds, pour off the alcohol, and then soak in 0.1% (mass volume ratio) mercury solution for disinfection for 10 minutes or 15 minutes or 20 minutes. During the disinfection, shake the jar constantly to ensure the integrity of the disinfection process. Evenly and thoroughly. Then take out the bud body and place it in another sterilized jar and wash it 6-8 times with sterile water to obtain the inocul...

Embodiment 2

[0027] A method for rapid propagation of overwintering buds of Epimedium sagittatum, the steps of which are as follows:

[0028] 1) Disinfection of explants: take the tiller buds formed in the fall of Epimedium that year as overwintering buds. Scrub the surface of the overwintering buds clean, then rinse them under running water for 60 minutes, and dry them until there is no water stain on the surface of the buds. (volume ratio) alcohol solution for 40s, pour off the alcohol, and then immerse and disinfect with 0.1% (mass volume ratio) mercuric acid solution for 15 minutes. During the disinfection, shake the jar constantly to ensure that the disinfection process is even and thorough. Then take out the bud body and place it in another sterilized jar and wash it 6-8 times with sterile water to obtain the inoculation material;

[0029]2) Callus induction: After the buds are sterilized, the outer bracts are cut off, and the internally differentiated buds are cut into small pieces...

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Abstract

The invention discloses a method for rapidly reproduction overwintering buds of epimedium sagittatum. According to the method, overwintering buds of epimedium sagittatum are taken as explants and are subjected to tissue culture, induced differentiation and rooting reproduction after being sterilized. The differentiation rate of callus differentiation buds is as high as 88.9%, the culture medium does not need to be replaced in later enrichment culture, and the multiplication coefficient is up to 5.3. The method is simple to operate, high in reproduction rate, low in production cost, short in reproduction period and applicable to factory production and application.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for rapid propagation of overwintering buds of Epimedium sagitta. Background technique [0002] Epimedium sagittatum is a perennial herbal medicinal plant of the genus Epimedium sagittarius in the family Berberidaceae. It is a traditional tonic Chinese medicine in my country. It has a cultivation history of more than 2,000 years and is one of the most widely used and longest traditional Chinese medicines in China. one. The traditional propagation methods of Epimedium sagitta are vegetative propagation through underground rhizomes and seed propagation. Seed reproduction has a low germination rate and slow growth. Therefore, the asexual reproduction method of ramet propagation is mostly used in production. These methods are strictly limited by the growing season and climate, resulting in low reproduction coefficient, which limits the industrialization and co...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王瑛吕海燕黄文俊
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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