A kind of epimedium sagittarius embryo tissue culture rapid propagation method

A technology for tissue culture, rapid propagation and epimedium, which is applied in the field of plant tissue culture to achieve the effects of simplifying operation, improving ratio and simple operation.

Active Publication Date: 2022-03-08
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are relatively few reports on the tissue culture technology of Epimedium sagittarius, and there are few reports on the use of epimedium sagittarius embryos to induce dedifferentiation of callus and then differentiate into sterile seedlings for plant tissue culture. Huohu breeding technology laid a solid foundation

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  • A kind of epimedium sagittarius embryo tissue culture rapid propagation method
  • A kind of epimedium sagittarius embryo tissue culture rapid propagation method

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Effect test

Embodiment 1

[0048] Embodiment 1: a kind of Epimedium sagittatum embryo tissue culture rapid propagation method, its steps are as follows:

[0049] (1) Disinfection of dried seeds: Select healthy and plump seeds from the seed collection bag after removing grass leaves and other impurities, tear off the seed film on the seeds, wash with detergent solution at a ratio of 1:500, and place Shake in a shaking box at 120rpm for 1 hour to remove the dust attached to the surface, rinse with clean water 3 or 4 times to remove residual detergent; move it into an ultra-clean workbench, then shake and disinfect with 75% alcohol solution for 30 seconds, rinse with sterile water 2 times, put the seeds in 0.1% HgCl 2 In the solution, shake and disinfect at 120rpm for 10min, take it out and rinse it with sterile water for 3 or 4 times to remove residual HgCl 2 solution; then place it on sterilized filter paper to remove residual water for later use.

[0050] (2) Seed low-temperature cultivation: inoculat...

Embodiment 2

[0056] Embodiment 2: a kind of Epimedium sagittatum embryo tissue culture rapid propagation method, its steps are as follows:

[0057] (1) Disinfection of dried seeds: Select healthy and plump seeds from the seed collection bag after removing grass leaves and other impurities, tear off the seed film on the seeds, wash with detergent solution at a ratio of 1:500, and place Shake in a shaking box at 120rpm for 1 hour to remove the dust attached to the surface, rinse with clean water 3 or 4 times to remove residual detergent; move it into an ultra-clean workbench, then shake and disinfect with 75% alcohol solution for 30 seconds, rinse with sterile water 2 times, put the seeds in 0.1% HgCl 2 In the solution, shake and disinfect at 120rpm for 10min, take it out and rinse it with sterile water for 3 or 4 times to remove residual HgCl 2 solution; then place it on sterilized filter paper to remove residual water for later use.

[0058] (2) Seed low-temperature cultivation: inoculat...

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Abstract

The invention relates to the technical field of plant tissue culture, and specifically discloses a method for tissue culture and rapid propagation of Epimedium sagittatum embryos. Regenerated seedlings of Epimedium sagittarius were obtained through low temperature culture, induction of explants to generate callus, induction of callus to differentiate into adventitious buds or clustered buds, induction of buds to form fibrous roots, seedling hardening, and seedling cultivation, and the establishment of Epimedium sagittarius Rapid propagation system of sheepskin plant tissue culture. It has the advantages of simple operation, low pollution rate, short reproduction cycle, high reproduction rate, low cost, and high survival rate of seedlings in the field. It provides technical support for the industrial production of Epimedium sagittarius and protects wild resources of Epimedium sagittarius There is a certain promotion effect.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, and more specifically relates to a method for tissue culture and rapid propagation of epimedium sagittarius embryos. Background technique [0002] Epimedium, a traditional Chinese medicine, is the dried leaf of Epimedium in the Berberidaceae family (Berberidaceae), and has been used as a medicinal material in my country for more than 2,000 years. At present, the acquisition of Epimedium medicinal materials mainly relies on wild picking. Extinct mining has drastically reduced the wild resources of Epimedium medicinal materials, and the contradiction between supply and demand has become increasingly serious. Epimedium sagittatum (Sieb.et Zucc.) Maxim. is one of the basic plants of Epimedium sagittatum in the "Chinese Pharmacopoeia", and it is widely used. At present, the artificial planting of this species has been carried out in many provinces stand up. Seedling breeding technology ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 梁琼张燕君于淑霞黄天悦于东悦王兰香
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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