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A method for rapid propagation of medicinal mulberry winter bud tissue culture regeneration

A technology of tissue culture and winter buds, which is applied in the field of tissue culture regeneration and rapid propagation of mulberry winter buds, which can solve the problems of low winter bud culture regeneration rate, subculture proliferation coefficient and root induction rate, long subculture proliferation time and induced rooting time, and leaf change. Huang and other problems, to achieve the effect of short breeding cycle, low production cost and shortened time

Active Publication Date: 2022-04-12
INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Wang Qianling et al. (2012), Ding Tianlong et al. (2013), Zeng Qiwei et al. (2016) and Li Zhengang et al. (2018) used MS as the basic medium and 6-BA and NAA as the main proliferative hormones to preliminarily establish the in vitro production of Sangdong buds. Tissue culture regenerative propagation system, the obtained mulberry tissue culture seedlings were transplanted and survived in Chongqing area and Qujing area, but the regeneration rate, subculture proliferation coefficient and root induction rate of winter buds were low
Wei Jia et al. (2015) used aseptic tissue cultured seedlings of mulberry as the test material to further optimize the subculture proliferation medium and root induction medium. The value-added coefficient reached 6.8 and the rooting rate reached 95%. It takes a long time to induce rooting, usually 45 days, and there are still problems such as fewer roots and yellow leaves

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  • A method for rapid propagation of medicinal mulberry winter bud tissue culture regeneration

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Embodiment 1

[0031] A method for rapid propagation of medicinal mulberry winter bud tissue culture regeneration, the steps are as follows:

[0032] 1) Disinfection of explants: During the period from the fall of leaves in autumn to the germination of the next spring, the buds on the 1-year-old branches of the medicinal mulberry are taken, that is, the winter buds. Scrub the surface of the bud body, then rinse it under running water for 30-60 minutes, and dry it until there is no water stain on the surface of the bud body. (volume ratio) alcohol solution for 30s, shake continuously during the period, pour off the alcohol, then soak and disinfect with 1.0g / L mercuric acid solution for 8-10min, shake the jar constantly during disinfection to ensure uniform and thorough disinfection process. Then take out the bud body and place it in another sterilized jar and wash it 6-8 times with sterile water to obtain the material to be inoculated;

[0033] 2) Primary culture: After the buds are steriliz...

Embodiment 2

[0046] 1) Disinfection of explants: During the period from the fall of leaves in autumn to the germination of the next spring, the buds on the 1-year-old branches of the medicinal mulberry are taken, that is, the winter buds. Scrub the surface of the bud body, then rinse it under running water for 30-60 minutes, and dry it until there is no water stain on the surface of the bud body. (volume ratio) alcohol solution for 30s, shake continuously during the period, pour off the alcohol, then soak and disinfect with 1.0g / L mercuric acid solution for 8-10min, shake the jar constantly during disinfection to ensure uniform and thorough disinfection process. Then take out the bud body and place it in another sterilized jar and wash it 6-8 times with sterile water to obtain the material to be inoculated;

[0047] 2) Primary culture: After the buds are sterilized, gently peel off the outer coating scale until the green true leaves are exposed, and the buds are 0.3cm-0.5cm in size, and pl...

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Abstract

The invention belongs to the technical field of plant tissue culture, and discloses a rapid propagation method for tissue culture, regeneration and reproduction of Sangdong bud. In the invention, the medicinal mulberry bud is used as the explant, DKW is used as the basic medium, ZT and IBA are added as the main plant hormones, and the rapid propagation technology of the medicinal mulberry bud in vitro regeneration is established. 35 days after inoculation, the proliferation coefficient could reach 6.8-7.3, and the regeneration rate of cluster buds was 86.7%-93.3%. The adventitious root regeneration rate can reach 92% to 96.5%, and the average number of adventitious roots is 4.6; by using the method of the invention, the cultivation time of the medicinal mulberry is significantly shortened, and the root system of the obtained adventitious root sterile seedlings is developed at the same time, and it is not necessary to refine the seedlings in the tissue culture room. , the slow seedling period is short, the survival rate after transplanting is high, and it is suitable for large-scale promotion.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for tissue culture regeneration and rapid propagation of medicinal mulberry winter buds. Background technique [0002] In plant taxonomy, medicinal mulberry belongs to Rosales (Rosales), Moraceae (Moraceae), Morus (Morus) and black mulberry species (Morus nigra L.). According to the latest mulberry chromosome base research results, Yaomulus is a natural polyploid plant with the highest chromosomal ploidy among flowering plants, and the chromosomal ploidy is twenty-docoploid (2n=22x=308) (Jiao et al., 2020 ). Medicinal mulberry is originally produced in Iran and was introduced to the southern Xinjiang of China in the 16th century. The fruit and leaves of medicinal mulberry have high nutritional and medicinal value. Uighurs are accustomed to using the fruit of mulberry to treat inflammation such as tonsillitis, sore throat and anemia. syndrome, e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 莫荣利李勇于翠董朝霞朱志贤邓文胡兴明
Owner INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
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