Medicinal mulberry winter bud tissue culture regeneration rapid propagation method

Abstract

The invention belongs to the technical field of plant tissue culture, and discloses a medicinal mulberry winter bud tissue culture regeneration rapid propagation method. According to the method, a medicinal mulberry winter bud in-vitro regeneration rapid propagation technology is established by taking a medicinal mulberry winter bud as an explant, DKW as a basic culture medium and ZT and IBA as main plant hormones, the callus formation rate of the medicinal mulberry winter bud in primary culture is 100%, the propagation coefficient can reach 6.8-7.3 after inoculation for 35 days, and the regeneration rate of cluster buds is 86.7%-93.3%; and the regeneration rate of adventitious roots can reach 92%-96.5%, and the average number of the adventitious roots is 4.6. By utilizing the method disclosed by the invention, the medicinal mulberry culture time is remarkably shortened, and meanwhile, obtained adventitious root aseptic seedlings have developed root systems, do not need to be acclimatized and domesticated in a tissue culture room, and are short in rejuvenation period, high in survival rate after transplanting and suitable for large-scale popularization.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for tissue culture regeneration and rapid propagation of medicinal mulberry winter buds. Background technique [0002] In plant taxonomy, medicinal mulberry belongs to Rosales (Rosales), Moraceae (Moraceae), Morus (Morus) and black mulberry species (Morus nigra L.). According to the latest mulberry chromosome base research results, Yaomulus is a natural polyploid plant with the highest chromosomal ploidy among flowering plants, and the chromosomal ploidy is twenty-docoploid (2n=22x=308) (Jiao et al., 2020 ). Medicinal mulberry is originally produced in Iran and was introduced to the southern Xinjiang of China in the 16th century. The fruit and leaves of medicinal mulberry have high nutritional and medicinal value. Uighurs are accustomed to using the fruit of mulberry to treat inflammation such as tonsillitis, sore throat and anemia. syndrome, e...

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Problems solved by technology

Wang Qianling et al. (2012), Ding Tianlong et al. (2013), Zeng Qiwei et al. (2016) and Li Zhengang et al. (2018) used MS as the basic medium and 6-BA and NAA as the main proliferative hormones to preliminarily establish the in vitro production of Sangdong buds. Tissue culture regenerative propagation system, the obtained mulberry tissue culture seedlings were transplanted and survived in Chongqing area and Qujing area, but the regeneration rate, subculture proliferation coefficient and root induction rate of winter buds were low

Wei Jia et al. (2015) used aseptic tissue cultured seedlings of mulberry as the test material to further optimize the subculture proliferation medium and root induction medium. The value-added coefficient reached 6.8 and the rooting rate reached 95%. It takes a long time to induce rooting, usually 45 days, and there are still problems such as fewer roots and yellow leaves

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