Blood cell analyzer, reticulocyte counting device and counting correction method

Abstract

A blood cell analyzer, a reticulocyte counting apparatus and a count correcting method are disclosed. The count correcting method comprises following steps: (1) carrying out a reaction between a fluorescent reagent and a blood sample to obtain a sample liquid; (2) feeding a proper amount of the sample liquid to an optical detection device for carrying out light irradiation and detecting optical information generated by various cells particles in the sample liquid; (3) obtaining an original reticulocyte counting value according to the optical information generated by the various cell particles, wherein the original reticulocyte comprises reticulocyte and leukocyte; and (4) performing the original reticulocyte counting value minus the leukocyte counting value to obtain a corrected reticulocyte counting value. By means of correction to the reticulocyte counting value by the leukocyte counting value, influences on count of the reticulocyte due to immature granulocyte or archaeocyte in the leukocyte is reduced and accuracy of the reticulocyte counting value is improved.

Description

technical field [0001] The present application relates to the field of blood cell analysis, in particular to a blood cell analyzer, a reticulocyte counting device and a counting correction method thereof. Background technique [0002] Reticulocytes (RET) are transitional cells between late immature erythrocytes and mature erythrocytes, slightly larger than mature erythrocytes. Reticulocytes are the stage when nucleated red blood cells have just lost their nuclei. They are still immature red blood cells. There are still basophilic substances such as ribosomes and ribonucleic acid in the cytoplasm. They are stained with brilliant tar blue or new methylene blue in vivo. Finally, blue or blue-green dendritic or even reticular structures can be seen in the cytoplasm, hence the name reticulocytes. Reticulocytes are an important stage in the maturation of red blood cells. [0003] In recent years, instruments that use flow analysis technology for RET counting have come out one af...

AI Technical Summary

Problems solved by technology

However, the inventor found in the research on the accurate counting of reticulocytes and the method of accurately measuring the reticulocyte maturation index that in the blood of some patients, there will be immature granulocytes or blasts in the white blood cells. Due to the staining efficiency, they are different from the The fluorescence intensity of the reticulocytes is close to that of the reticulocytes, which may enter the sensitive area of ​​the reticulocytes, eventually leading to a false high count of reticulocytes, such as figure 2 , 3 shown

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