Breast cancer early stage diagnosis sialoprotein fingerprint model and construction method thereof

Abstract

The invention discloses a construction method of a breast cancer early stage diagnosis sialoprotein fingerprint model. The construction method of the breast cancer early stage diagnosis sialoprotein fingerprint model comprises the following steps: collecting saliva of a breast cancer patient and a health control, centrifuging,a nd combining with an NP20 protein chip; reading the chip by adopting a PBSII protein chip reader to obtain a preliminarily screened result, and carrying out burst data means comparing rank-sum test on the preliminarily screened mass-to-charge ratio peak; and carrying out data analysis through calculating the classification values of many variable changes on two samples via a decision tree algorithm by Biomaker Pattern Software. The breast cancer early stage diagnosis sialoprotein fingerprint model comprises human salivay proteins with the mass-to-charge ratio (m / z) of 4849.31, 5224.96, 3439.02 and 3559.89 respectively. The breast cancer diagnosis can be preliminarily realized by analyzing the m / z and model of corresponding proteins in human saliva, the predication accuracy is 78.02%, and the construction method has the advantages of reasonability, feasibility, simple operation and batch treatment.

Description

technical field [0001] The invention belongs to the technical field of breast cancer detection, in particular to a salivary protein fingerprint model for early diagnosis of breast cancer and a construction method thereof. Background technique [0002] Breast cancer is the most common malignancy in women. In recent years, the incidence of breast cancer in China, especially in developed regions, has shown a rapid growth trend, and has leapt to the top of the incidence of female malignant tumors. However, there is currently no effective means for the early diagnosis of breast cancer clinically. Saliva has special advantages in disease diagnosis due to its non-invasive method of body fluid collection. As early as 1901, Michaels took the lead in using saliva composition as an auxiliary tool for disease diagnosis. Now the value of saliva as a disease diagnosis specimen has received unprecedented attention . Saliva is an indispensable and important body fluid for the human body....

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Problems solved by technology

[0003] The purpose of the embodiments of the present invention is to provide a salivary protein fingerprint model for early diagnosis of breast cancer and its construction method, aiming to solve the problem of lack of diagnostic ability and classification detection model for early diagnosis of breast cancer

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