Method for rapidly creating sweet-waxy recessive maize inbred line

Abstract

The invention discloses a method for rapidly creating a sweet-waxy recessive maize inbred line, and specifically relates to a method for rapidly creating a sweet-waxy recessive maize homozygous inbred line by combining a haploid inducing technology and a molecular marker technology. The method comprises the following steps: hybridizing sweet maize and waxy maize, collecting F1 seeds, hybridizing F1 as a female parent and a haploid inducing line with a purple marker to obtain haploid seeds, obtaining powder, waxy and sweet maize double haploid lines by haploid doubling and inbreeding, identifying a sweet material by using a molecular marker linked with a wx gene to obtain a sweet-waxy recessive inbred line material. The 100% genetic homozygous sweet-waxy recessive inbred line can be obtained only through three generations in the method provided by the invention. The method provided by the invention has the advantages of short selective breeding cycle, wide selection of base materials, small field trial scale, low cost, fully homozygous inbred line and stable and unseparated characters.

Description

technical field [0001] The invention belongs to the technical field of breeding of new agricultural varieties, in particular to a method for quickly creating a homozygous inbred line of sweet and waxy double recessive maize. Background technique [0002] With the continuous expansion of the demand for fresh corn and the continuous expansion of consumer groups, people's demand for new types of fresh corn that is sweet and waxy, and waxy and sweet is increasing rapidly. Breeders' efforts. Genetic studies have shown that both the sweet quality gene and the waxy quality gene are recessive, and the sweet quality is epistatic to the waxy quality, that is, the phenotype of the homozygous sweet and waxy quality is sweet quality. Therefore, the same grain can only be sweet or waxy. By crossing the sweet and waxy double recessive inbred line with the waxy homozygous inbred line, the F2 ear of the F1 plant will be sweet and waxy at a ratio of 1:3. The grain separation ratio, the tast...

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Problems solved by technology

For example, the breeding method of Wu Zikai of Guangxi University is to cross the two different types of single recessive gene homozygous corns of sweet and waxy according to the segregation and independent distribution of genes, and select the double recessive gene homozygous kernels on the ears of F1 plants. , a stable sweet-waxy double-recessive corn inbred line was obtained through multiple generations of selfing. Bagging self-crossing generally takes more than 8 generations, and in addition to the previous process of crossing and selection of different types of corn, a homozygous sweet-waxy double-recessive corn inbred line was bred. Waxy double-recessive corn inbred lines need at least 10 generations; each generation needs to be bagged and inbred, which requires a lot of work and high cost

Xu Yong from Nanjing Agricultural University and Dai Huixue from Nanjing Institute of Vegetable Science conducted multi-generation recurrent selection based on kernel color. Although their method can breed sweet and glutinous double recessive maize inbred lines, color selection has a great influence. Limitations, and the workload of multi-generation reincarnation selection is also very large

Meng Qingchang of the Jiangsu Academy of Agricultural Sciences also selects according to the color of the seeds. Although the backcross method can shorten the breeding time, it takes more than 7 generations to breed the sweet and glutinous double recessive inbred line.

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