Breeding method of two-line CMS line with variegated leaves, long stigma, long panicle neck and bare body
A technology with variegated leaves and long panicle necks, which is applied in the fields of botany equipment and methods, applications, plant genetic improvement, etc., can solve the problems of low purity of hybrids, high risk factor of seed production, poor purity, etc., and achieve high purity , easy mechanized harvesting and cost reduction
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Embodiment 1
[0017] 1) Take rice striped leaf mutant st(t) (A) (Sang Xianchun et al. Identification and genetic mapping of rice striped mosaic mutant st(t). Acta Crops, 2010,36(2):211 ?216), the long stigma variety Hai B (B) (Chen Yuxin. Breeding research on the long stigma male sterile line Hai A of hybrid rice. Journal of Hainan University, Natural Science Edition, 1991,9(2):54-58), Changsui The neck variety Shuhui 527eR(1) (C) and the naked rice variety Lemont (D) were used as parental materials, and the two-line male sterile line C815S was used as the recurrent parent (R) to cross A / B and C / D to obtain a hybrid f 1 Substituted by F 1-AB and F 1-CD express;
[0018] 2) Planting hybrid F 1 Substitute material F 1-AB and F 1-CD , in the flowering stage for hybrid F 1-AB / F 1-CD , get recrossed 1 generation, with F 1-ABCD express;
[0019] 3) Planting step 2) obtained recross F 1-ABCD , F 2-ABCD , planting F 2-ABCD , with a population of 1,600 plants. At the seedling stage, id...
Embodiment 2
[0026] 1) The rice striped leaf mutant st(t) (A), the long stigma variety HaiB (B), the long panicle neck variety Shuhui 527eR(1) (C), and the naked rice variety Lemont (D) were used as parents Materials, using the two-line male sterile line Y58S as the recurrent parent (R), cross A / B and C / D, and get the cross F 1 Substituted by F 1-AB and F 1-CD express;
[0027] 2) Planting hybrid F 1 Substitute material F 1-AB and F 1-CD , in the flowering stage for hybrid F 1-AB / F 1-CD , get recrossed 1 generation, with F 1-ABCD express;
[0028] 3) Planting step 2) obtained recross F 1-ABCD , F 2-ABCD , planting F 2-ABCD , with a population of 1,700 plants. At the seedling stage, identify and select rice seedlings with both variegated leaves and bare body traits and transplant them to the field. At the flowering stage, select 3 rice plants with both long stigma and long panicle neck traits and Y58S (as the female parent) Hybrid, get hybrid F 1 Generation, to RF 1 express; ...
Embodiment 3
[0035] 1) The rice striped leaf mutant st(t) (A), the long stigma variety HaiB (B), the long panicle neck variety Shuhui 527eR(1) (C), and the naked rice variety Lemont (D) were used as parents The material is two-line male sterile line quasi-S as the recurrent parent (R), and crosses A / B and C / D to obtain the cross F 1 Substituted by F 1-AB and F 1-CD express;
[0036] 2) Planting hybrid F 1 Substitute material F 1-AB and F 1-CD , in the flowering stage for hybrid F 1-AB / F 1-CD , get recrossed 1 generation, with F 1-ABCD express;
[0037] 3) Planting step 2) obtained recross F 1-ABCD , F 2-ABCD , planting F 2-ABCD , with a population of 1800 plants. At the seedling stage, identify and select rice seedlings with both striped leaves and bare body traits to transplant to the field. At the flowering stage, select 3 rice plants with both long stigma and long panicle neck traits and quasi-S (as the female parent ) to cross, get hybrid F 1 Generation, to RF 1 express;...
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