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A method of using pfu to collect microbiological communities to degrade petroleum

A technology of microbial communities and micro organisms, which is applied in the field of oil pollution in waters degraded by micro-biological communities, can solve the problems of easy degradation of strains, cumbersome screening steps, and reduced oil degradation ability, and achieve easy acquisition, low cost, and shortened cycle Effect

Inactive Publication Date: 2016-11-02
HEBEI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current research methods of bioremediation are all aimed at screening oil-degrading bacteria, and then immobilizing them, and then using them to degrade oil. The screening steps are cumbersome, and some bacteria are prone to degradation, and their ability to degrade oil will gradually decrease.

Method used

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  • A method of using pfu to collect microbiological communities to degrade petroleum
  • A method of using pfu to collect microbiological communities to degrade petroleum
  • A method of using pfu to collect microbiological communities to degrade petroleum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Degradation of Crude Oil by Microbial Community

[0031] The microbiological community was collected in Huanghuagou, Baoding, a flowing river, and the collection time was April 2010.

[0032] Before collecting, first check the integrity of the micro-biological community in the sampled waters. The specific method: use No. 25 plankton net to sample, and check the sample with a laboratory microscope after collection. Algae, flagellates, sarcoids, ciliates, etc. should be observed in the samples Microorganisms.

[0033] The initial microbiological community structure of Huanghuagou is shown in Table 1.

[0034] Table 1

[0035]

[0036]

[0037] It can be seen from Table 1 that the microbiological community in Huanghuagou at this time is complete, including all major microbiological groups, and is suitable for collection.

[0038] a. Cut the PFU into a 1cm×1cm×1cm square, soak it in physiological saline at 20°C for 24h, take it out and rinse it with steri...

Embodiment 2

[0046] The microbiological community was collected in April 2010 under the Anxin Bridge in Baiyangdian, Baoding, which is a still water body.

[0047] Before collecting, first check the integrity of the micro-biological community in the sampled waters. The specific method: use No. 25 plankton net to sample, and check the sample with a laboratory microscope after collection. Algae, flagellates, sarcoids, ciliates, etc. should be observed in the samples Microorganisms.

[0048] Table 3 shows the initial microbiological community structure of Anxin Bridge lake water.

[0049] table 3

[0050]

[0051] It can be seen from Table 3 that the microbiological community in the lake under the Anxin Bridge at this time is complete, including all major microbiological groups, and is suitable for collection.

[0052] a. Cut the PFU into a square of 1cm×1cm×1cm, soak it in normal saline at 25°C for 24h, take it out and rinse it with sterile water for 3 times, squeeze out the water, and dr...

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Abstract

The invention discloses a method for degrading oil by means of microbial communities acquired by a PFU. According to the method, the microbial communities, existing in the water areas such as rivers and lakes, acquired by a PFU block with the size of 1cm*1cm*1cm under the natural condition are used for degrading oil or 20# heavy diesel oil, the degrading efficiency is high, degradation is avoided, the method is used for treating a water area which is polluted by oil or 20# heavy diesel oil, the effect is good, operation is easy, the cycle is short, cost is low, and secondary pollution to the environment is avoided.

Description

technical field [0001] The invention belongs to the technical field of biotechnological treatment of polluted water bodies, and in particular relates to a method for using PFU to collect microbiological communities under natural conditions to degrade polluted oil in water areas. Background technique [0002] Petroleum is mainly a mixture of various hydrocarbons (alkanes, cycloalkanes, aromatic hydrocarbons, polycyclic aromatic hydrocarbons) and a small amount of other organic compounds (sulfides, nitrides, alkane acids). With the rapid development of society and economy, Petroleum and its products are widely used in various fields of society. But at the same time, the pollution of oil to the environment has also become the focus of global attention. Oil pollution has caused serious harm to soil, air, groundwater, ocean, human beings, animals, and plants, especially the development of offshore oil exploration, development, and refining industries, and the occurrence of trans...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/00C02F3/32C02F3/34
CPCC02F3/325C02F3/344C02F2103/007
Inventor 李凤超康现江何炜王福强曹卫荣
Owner HEBEI UNIVERSITY
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