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Amplification primer and reaction system of spider mitochondrial genome control region

A technology of mitochondrial genome and amplification primers, applied in the direction of recombinant DNA technology, DNA / RNA fragments, etc., to achieve the effect of promoting development, important invention value and theoretical significance

Active Publication Date: 2017-07-14
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the studies on spider genetics and evolutionary biology using mitochondrial control region sequences are specific primers for a specific species, and there is no universal primer that can effectively amplify most spider mitochondrial genome control regions.

Method used

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  • Amplification primer and reaction system of spider mitochondrial genome control region

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Collection, identification and preservation of spider samples

[0021] The species of spiders used in the examples were all collected from the farmland of Yuyao City, Zhejiang Province by trap method. The collected specimens were brought back to the laboratory for information registration, and were identified with a stereo microscope to determine the species. The spider specimens used for molecular biology experiments were soaked in 100% alcohol and stored in a 4°C refrigerator for later use. The species of spiders collected include: Pseudomonas tarantula, Triflower spider, Golden spider, Cone-bellied octopus, Ornate octopus, Brown new orb spider, Maiwa spider, black fly tiger, big-bellied garden spider, cat jumping spider;

[0022] (2) Extraction of total DNA from the spider genome

[0023] DNA extraction kits were used to extract the total genomic DNA of different species of spiders. To avoid contamination, the head and abdomen were discarded, and DNA was extra...

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Abstract

The invention discloses an amplification primer of a mitochondrial genome control region of a spider and a reaction system of the amplification primer. The amplification primer consists of two single strain oligonucleotides, wherein a forward primer Ctrl-F is shown in SEQ ID NO: 1 and a reverse primer Ctrl-R is shown in SEQ ID NO: 2. The amplification primer disclosed by the invention can be used for quickly, efficiently and specifically amplifying the mitochondrial genome control regions of various spiders, has important meaning in the aspects of rapid identification of the types of the spiders, analysis of population diversity, protection and utilization of genetic resources and the like and further provides a powerful tool for analyzing and researching evolution of different taxonomic category systems of the spiders.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an amplification primer for a spider mitochondrial genome control region and a reaction system thereof. Background technique [0002] Spiders are a class of predatory arthropods that mainly prey on insects. They play a very important role in the biological control of pests and maintaining the balance of the ecosystem. Attention should be paid to protecting the natural enemy species pool and promoting the reconstruction of the natural enemy community, which can effectively control the occurrence and damage of many agricultural pests, such as planthoppers, leafhoppers, stem borers, and cotton aphids. There are many kinds of spiders, so it is extremely important to quickly and accurately identify species and carry out flora classification research, which is of great significance to the effective protection and full utilization of spider germplasm resources. [0003] Arthrop...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888
Inventor 王正亮俞晓平李超
Owner CHINA JILIANG UNIV