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Separating and purifying method for Chinese wolfberry polyose

A technology for separation and purification of Lycium barbarum polysaccharide is applied in the field of separation and purification of biological macromolecules.

Inactive Publication Date: 2015-04-29
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the defects of long process flow, high material consumption and energy consumption, and large pollution of the current Lycium barbarum polysaccharide purification process, and provide a separation and purification method of Lycium barbarum pure polysaccharide with simpler process flow, low cost, and environmental protection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) The new temperature-sensitive block copolymer polyethylene oxide-polypropylene oxide-polyethylene oxide (EOPOEO) was mixed with sodium dihydrogen phosphate to prepare an aqueous two-phase system of EOPOEO-sodium dihydrogen phosphate. The mass fraction of sodium dihydrogen phosphate in the aqueous two-phase system is 12%, and the mass fraction of EOPOEO is 20%;

[0025] (2) Add the wolfberry crude polysaccharide solution into the EOPOEO-sodium dihydrogen phosphate two-phase aqueous system described in step (1), shake and mix, so that the concentration of wolfberry polysaccharide in the system is 6mg / g, and then statically Place the phase separation for 12h, and separate the upper and lower layers;

[0026] (3) Put the lower phase obtained in step (2) into a dialysis bag (8000-14000Da), and dialyze in distilled water to remove salt and other small molecular impurities. Finally, the obtained dialysis product was mixed evenly with 5 times the volume of 95% ethanol, and...

Embodiment 2

[0029] (1) The new temperature-sensitive block copolymer polyethylene oxide-polypropylene oxide-polyethylene oxide (EOPOEO) was mixed with sodium dihydrogen phosphate to prepare an aqueous two-phase system of EOPOEO-sodium dihydrogen phosphate. The mass fraction of sodium dihydrogen phosphate in the aqueous two-phase system is 28%, and the mass fraction of EOPOEO is 20%;

[0030] (2) Add the wolfberry crude polysaccharide solution into the EOPOEO-sodium dihydrogen phosphate two-phase aqueous system described in step (1), shake and mix, so that the concentration of wolfberry polysaccharide in the system is 6mg / g, and then statically Place the phase separation for 12h, and separate the upper and lower layers;

[0031] (3) Put the lower phase obtained in step (2) into a dialysis bag (8000-14000Da), and dialyze in distilled water to remove salt and other small molecular impurities. Finally, the obtained dialysis product was mixed evenly with 5 times the volume of 95% ethanol, and...

Embodiment 3

[0034] (1) The new temperature-sensitive block copolymer polyethylene oxide-polypropylene oxide-polyethylene oxide (EOPOEO) was mixed with sodium dihydrogen phosphate to prepare an aqueous two-phase system of EOPOEO-sodium dihydrogen phosphate. The mass fraction of sodium dihydrogen phosphate in the aqueous two-phase system is 24%, and the mass fraction of EOPOEO is 12%;

[0035] (2) Add the wolfberry crude polysaccharide solution into the EOPOEO-sodium dihydrogen phosphate two-phase aqueous system described in step (1), shake and mix, so that the concentration of wolfberry polysaccharide in the system is 6mg / g, and then statically Place the phase separation for 12h, and separate the upper and lower layers;

[0036] (3) Put the lower phase obtained in step (2) into a dialysis bag (8000-14000Da), and dialyze in distilled water to remove salt and other small molecular impurities. Finally, the obtained dialysis product was mixed evenly with 5 times the volume of 95% ethanol, and...

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Abstract

The invention belongs to the technical field of biomacromolecule separation and purification, and particularly relates to a separating and purifying method for Chinese wolfberry polyose. According to the method provided by the invention, novel thermo-sensitive periodic copolymers, namely polyethylene oxide-polypropylene oxide-polyethylene oxide and inorganic salt, are used for forming a two-phase aqueous system, and small molecular weight impurities such as protein, coloring matters and the like in Chinese wolfberry polyose are removed. Inorganic salt in a lower phase is removed according to a dialysis method, and ethyl alcohol is deposited to obtain a polyose finished product; in virtue of a temperature response behavior of a thermo-sensitive polymer, and through multi-step comprehensive operations such as protein precipitation through temperature reduction, PH value adjustment, heating for phase separation and the like, the thermo-sensitive polymer in an upper phase can be recovered successfully. The invention provides a novel protein removing method which is efficient, green and low in cost, the thermo-sensitive periodic copolymer is effectively recovered and reused, the defects of high price and difficult recovering of a polymer formed through a traditional polymer aqueous two phase method can be overcome, material consumption and cost are reduced greatly, and the method can be applied to industrialized production in large scale.

Description

technical field [0001] The invention belongs to the technical field of separation and purification of biological macromolecules, and in particular relates to a separation and purification method of Lycium barbarum polysaccharide. Background technique [0002] Lycium barbarum polysaccharide is a macromolecular substance composed of several monosaccharides, with a molecular weight between 80 and 120 kDa. It is an important active substance in Lycium barbarum. Lycium barbarum polysaccharide has good health care and medical value, and has good functions in enhancing immune function, anti-aging, protecting liver, lowering blood sugar, lowering blood fat, inhibiting tumor growth, sensitizing radiotherapy, and synergizing and reducing toxicity of chemotherapy. Lycium barbarum polysaccharide shows broad commercial value due to its good health care and medicinal value. The traditional method of extracting polysaccharides from Lycium barbarum is mostly water extraction and alcohol pr...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 王赟胡晓玮韩娟倪良陈桐胡钰涛
Owner JIANGSU UNIV
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