Synergistic elution and remediation method for tetracycline and heavy metal compound high-abundant resistant gene contaminated site soil

A technology of resistance gene and repair method, which is applied in the restoration of polluted soil, etc., can solve the problems of less soil attention, poor repair effect, poor environmental friendliness of chemical eluent, etc., and achieves wide application prospects and easy operation , easy to promote the effect

Active Publication Date: 2015-05-06
INST OF SOIL SCI CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method adopts high temperature treatment, anaerobic and hypoxic cultivation, high pH treatment and strong light treatment, and can reduce more than 90% of the tetracycline resistance gene pollution in livestock and poultry feces through continuous series or partial series operation. The method was only developed for the removal of the tetracycline resistance gene, without taking into account the combined pollution of tetracycline and heavy metals.
[0004]Through relevant literature review and patent search, no public publications on the synergistic elution remediation technology of tetracycline and heavy metal complex high-abundance resistance gene-contaminated soil were found And acceptance, the prior art closest to the present invention is surfactant elution technology, application number: 200710010441.X provides a kind of heavy metal polluted soil washing device and method thereof, but this method is only for heavy metal complex polluted soil Remediation, and lack of verification for tetracycline and heavy metal compound contaminated soil leaching repair effect; application number: CN201010558286.7 provides a method for leaching and repairing organic-inorganic compound polluted soil, the method mainly uses sodium lauryl sulfate and Diethylamine disuccinic acid leaching polluted soil, but the organic pollutants targeted by this method are mainly chlorobenzene and trichloroethylene organic pollutants, and the heavy metals targeted are mainly copper and chromium polluted soil, and chemical leaching is adopted It has potential secondary environmental risks to the remediated soil; application numbers: CN200810123622 and CN201210486441.8 respectively provide a method for leaching and remediating heavy metal-organic compound polluted soil and a method for polycyclic aromatic hydrocarbon and heavy metal compound polluted soil The chemical-microbial joint remediation method, both of which are aimed at the remediation of compound-contaminated soil of polycyclic aromatic hydrocarbons and heavy metals, lacks the effectiveness verification for the soil contaminated by tetracycline and multiple heavy metals with high-abundance resistance genes
[0005] The main defect in the existing technology is: lack of synergistic elution technology for high-concentration tetracycline and heavy metal compound high-abundance resistance gene contaminated soil at the same time, selection The environmental friendliness of the chemical eluent is poor, the effect of pollutant removal is not complete, and the restoration effect of the ecological function of the soil environment after elution is not ideal. not clear
The main reasons for [0006] defects are as follows: In recent years, many feed mills and livestock and poultry farms generally add A large number of tetracycline antibiotics containing heavy metals, many drugs containing tetracycline parent or metabolites are excreted and accumulated in the soil with livestock and poultry manure. Due to the failure to timely and effectively deal with the excrement of such livestock and poultry manure, a large number of antibiotics have appeared. The tetracycline and heavy metal compound polluted soil near rural areas or suburban farms in China; early scientific and technological personnel paid little attention to this kind of contaminated soil, especially the high-abundance resistance genes induced under the conditions of tetracycline and heavy metal compound pollution Less attention has been paid to the new type of soil pollution, so the research and development of the available targeted remediation technologies are seriously lagging behind; the existing remediation technologies are mainly aimed at the farmland soil with relatively low pollution concentration, while for the site soil with high pollution concentration, remediation The effect is not good; the existing restoration technology has little consideration for the restoration of the diversity and stability of microbial ecological functions in the soil environment after restoration

Method used

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  • Synergistic elution and remediation method for tetracycline and heavy metal compound high-abundant resistant gene contaminated site soil
  • Synergistic elution and remediation method for tetracycline and heavy metal compound high-abundant resistant gene contaminated site soil
  • Synergistic elution and remediation method for tetracycline and heavy metal compound high-abundant resistant gene contaminated site soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] For total tetracycline concentrations greater than 1 mg kg -1 and less than 10 mg kg -1 , Cu concentration greater than 50 mg kg -1 And less than 100 mg kg -1 , Zn concentration greater than 50 mg kg -1 And less than 100 mg kg -1 , the abundance of tetracycline resistance gene is greater than 1×10 5 less than 1×10 6 Copy number of composite contaminated soil:

[0033] The test soil was collected from a site polluted by the accumulation of feces from a pig farm in Taizhou City, Jiangsu Province. The soil samples were air-dried, the impurities were sorted out, and the soil samples were passed through a 10-mesh sieve for later use. The basic physical and chemical properties of the soil are pH 6.6, organic matter content 47.3 g kg -1 , total nitrogen 3.6 g kg -1 , available nitrogen 89.5 mg kg -1 , total phosphorus 0.9 g kg -1 , available phosphorus 85.4 mg kg -1 . The mechanical composition of the soil is 5.3 wt.% sand, 61.8 wt.% loam, and 32.9 wt.% clay. The...

Embodiment 2

[0036] For total tetracycline concentrations greater than 10 mg kg -1 and less than 50 mg kg -1 , Cu concentration greater than 100 mg kg -1 And less than 200 mg kg -1 , Zn concentration greater than 100 mg kg -1 And less than 200 mg kg -1 , the abundance of tetracycline resistance gene is greater than 1×10 6 less than 1×10 7 Copy number of composite contaminated soil:

[0037] The test soil was collected from the polluted soil of a broiler farm in Bengbu City, Anhui Province. The soil samples were air-dried, the impurities were sorted out, and the soil samples were passed through a 10-mesh sieve for later use. The basic physical and chemical properties of the soil are pH 6.9, organic matter content 26.6 g kg -1 , total nitrogen 3.1 g kg -1 , available nitrogen 58.7 mg kg -1 , total phosphorus 0.9 g kg -1 , available phosphorus 61.3 mg kg -1 . The mechanical composition of the soil is 4.2 wt.% sand, 53.6 wt.% loam, and 42.2 wt.% clay. The background contamination...

Embodiment 3

[0040] For total tetracycline concentrations greater than 50 mg kg -1 , Cu concentration greater than 200 mg kg -1 , Zn concentration greater than 200 mg kg -1 , the abundance of tetracycline resistance gene is greater than 1×10 7 Copy number of composite contaminated soil:

[0041] The test soil was collected from the polluted soil of a poultry farm in Ningbo City, Zhejiang Province. The soil samples were air-dried, the impurities were sorted out, and the soil samples were passed through a 10-mesh sieve for later use. The basic physical and chemical properties of the soil are pH 6.1, organic matter content 15.7 g kg -1 , total nitrogen 1.2 g kg -1 , available nitrogen 34.1 mg kg -1 , total phosphorus 0.3 g kg -1 , available phosphorus 42.4 mg kg -1 . The mechanical composition of the soil is 7.8 wt.% sand, 58.1 wt.% soil, and 34.1 wt.% clay. The background contamination concentrations of total tetracycline, Cu, Zn and tetracycline resistance gene in soil were 61.44 ...

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Abstract

The invention provides a synergistic elution and remediation method for tetracycline and heavy metal compound high-abundant resistant gene contaminated site soil. The synergistic elution and remediation method comprises the following steps: firstly, crushing and screening the contaminated soil particles; placing the soil particles into a soil multielemental ex-situ remediation equipment, adding grass carp sperm DNA powder as an eluting liquid, and performing continuous ex-situ synergistic elution by adopting ultrasonic enhancement and temperature rise processing mode; applying a nutrient source buffer solution the mass of which is 10-20% that of the soil; and artificially culturing growth to complete remediation. According to the method, tetracycline, related tetracycline-resistant genes and various heavy metals in the soil can be simultaneously eluted and removed; the added foreign nutrition substances facilitate the rapid recovery of the diversity and stability of environmental microorganism ecological functions of eluted soil, and are capable of effectively alleviating the abundance secondary promotion and diffusion of the tetracycline-resistant genes in the soil.

Description

technical field [0001] The invention belongs to the technical field of remediation of organic-inorganic composite polluted soil, and in particular relates to a synergistic elution repair method of tetracycline and heavy metal composite high-abundance resistance gene polluted soil. Background technique [0002] In recent years, with the rapid development of my country's economy and the continuous improvement of people's living standards, the demand for livestock and poultry supply has also increased year by year. Therefore, driven by economic interests, many feed factories and large-scale farms generally add a large amount of tetracycline antibiotics containing heavy metals to the feed in order to promote the rapid growth of animals and prevent the outbreak of diseases. Due to the low bioavailability of livestock and poultry to absorb and utilize tetracycline in vivo, most tetracyclines are often directly or indirectly entered into the soil environment by livestock and poul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/02
Inventor 叶茂谢珊妮吕正勇蒋新程吟文张厦卞永荣谷成刚宋洋
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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