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Preparation method and anti-allergic and antioxidant activity detections of rutin and isoquercitrin in hops

An antioxidant activity, isoquercitrin technology, applied in the preparation of sugar derivatives, chemical instruments and methods, color/spectral property measurement, etc., to achieve good medicinal value and development prospects, high yield and purity, and rich sources Effect

Inactive Publication Date: 2015-05-06
SHANDONG UNIV
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Problems solved by technology

[0006] Hops contain a large amount of flavonoid glycosides, but there are no domestic reports on the preparation methods of flavonoid glycosides (rutin, isoquercitrin) in hops and the detection of anti-allergic and antioxidant activities

Method used

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Embodiment Construction

[0022] Now, the present invention will be further described in conjunction with the embodiments of the present invention and related tests.

[0023] Preparation of Rutin and Isoquercitrin from Hops

[0024] (1) Raw material extraction: crush 1kg of hops into coarse powder, add 5L of cold water to soak, 4 hours later, ultrasonic extraction once, ultrasonic time is 30min; extract. Extract twice by this method, combine the extracts, filter to obtain a clarified extract, and concentrate under reduced pressure;

[0025] (2) Preliminary removal of impurities: extract the concentrated extract with petroleum ether and ethyl acetate in sequence, wherein petroleum ether is extracted once and ethyl acetate is extracted three times. Fat-soluble components such as chlorophyll, large polar flavonoid aglycones, and small polar flavonoid glycosides are removed in sequence. Then extract the extract with n-butanol to obtain n-butanol extract, concentrate under reduced pressure at 45°C to obt...

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Abstract

The invention relates to a preparation method and anti-allergic and antioxidant activity detections of rutin and isoquercitrin in hops. With the method provided by the invention, problems in extracting and separating rutin and isoquercitrin with high anti-allergic and anti-oxidation properties from hops are effectively solved. According to the method, hops are adopted as a raw material, and are subjected to cold water soaking, extraction and impurity-removing, and macroporous resin separation and enrichment; and with a semi-preparative high-performance liquid chromatography technology, rutin and isoquercitrin monomeric compounds are obtained by separation; and the anti-allergic and antioxidant functions of rutin and isoquercitrin are determined with a hyaluronidase in-vitro inhibition method and a DPPH method. The method provided by the invention is advanced and reliable, and is easy to operate. With the method, high-purity rutin and isoquercitrin can be obtained. As a result of detections, rutin and isoquercitrin both have good anti-allergic and antioxidant activities, and have high application values.

Description

technical field [0001] The invention belongs to the field of medicine and natural product chemistry, and in particular relates to a preparation method of rutin and isoquercitrin in hops and detection of antiallergic and antioxidative activities. Background technique [0002] Hops is a perennial herbaceous plant of the Cannabis family Humulus genus, dioecious, mainly used in the beer brewing industry, but its medicinal value cannot be ignored. Its main active ingredients are resin components and their derivatives mainly composed of humulones and lupulones, polyphenolic compounds represented by xanthohumol and isoxanthohumol, and terpenes and terpenes Oxide-based volatile flavor components. Recent studies have shown that hop water extract is rich in flavonoid glycosides. [0003] Flavonoid glycosides are a large class of natural chemical products widely distributed in the plant kingdom. According to research reports, it can not only be used as a drug to prevent and treat ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08G01N21/31
CPCC07H17/07C07H1/08G01N21/3103
Inventor 怀其勇李阳马庆林
Owner SHANDONG UNIV
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