Adjustable Multilayer Cell Separation Sieve Device

Abstract

The invention discloses an adjustable multilayer cell separation sieve device which comprises a device main body, a motor, a bracket, a sieve and a limiting lever, wherein the device main body comprises a framework which consists of a stand column and a cross beam; a middle shaft is rotatably arranged at the middle position of the cross beam; a gearwheel and a hanging frame are arranged on the middle shaft; a small shaft is arranged at the end of the hanging frame; the bracket is suspended on the small shaft; a pinion is arranged at the bottom of the bracket and meshed with the gearwheel; the sieve comprises a sieve main body; a vertical sieve drum and a conical sieve screen are arranged in the sieve main body; and the limiting lever is hinged on the stand column. According to the adjustable multilayer cell separation sieve device provided by the invention, the centrifugal force generated along the axial direction of the sieve is used for separating the cells, the density of which is higher than that of the solution, and the centrifugal force generated along the radial direction of the sieve is used for separating the cells, the density of which is lower than that the solution. The adjustable multilayer cell separation sieve device is simple to operate, safe, low in cost, short in separation time, high in separated cell purity, free of cell damage deformation and suitable for popularization in clinic and scientific research fields.

Description

technical field [0001] The invention relates to the field of biotechnology, and more specifically relates to an adjustable multilayer cell separation sieve device. Background technique [0002] The development of science and technology in the field of life science reproduction and development plays an important role in supporting the improvement of human health and fertility. It is an important way for science and technology to benefit people's livelihood. It has become one of the key support areas of the national medium and long-term development plan. occupies an important position in the field. In modern society, infertility has become the third major disease after tumors and cardiovascular and cerebrovascular diseases. About 15% of couples in the world are troubled by infertility, and male and female factors account for half of it. According to reports, 90% of male infertility is caused by spermatogenesis disorders. Male infertility not only endangers male reproductive h...

AI Technical Summary

Problems solved by technology

With the help of centrifugal force, cells with different densities will form their own cell layers at the interface of adjacent Percoll gradients. The separation of different cell layers must be very careful, and a little carelessness will lead to remixing; obviously, this method is not accurate separation. technology, and the operating procedures are complicated, and the cell loss is large, so it is not suitable for batch separation of cells

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