Natural killer cell culture medium and natural killer cell amplification culture method
A natural killer cell, amplification and culture technology, applied in the direction of animal cells, vertebrate cells, blood/immune system cells, etc., can solve the problems of unreachable cell number, ineffective effect, low purity, etc., and achieve good killing activity, Improved amplification efficiency and high purity
Active Publication Date: 2015-05-06
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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Problems solved by technology
[0003] At present, most NK cell culture systems use fetal bovine serum plus basal medium for culture, but the use of fetal bovine serum for culture will have potential risks brought by heterologous serum
For this reason, it has also been reported that NK
Method used
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Embodiment 1
[0045] Embodiment 1 The present invention provides the preparation of culture medium
[0046] Mix 900mL LONZA X-VIVO 15 medium, 100mL human plasma, 1.0×10 5 U of IL-2, 5 μg of IL-21, 5 μg of IL-15 and 5 μg of OKT-3 were mixed and filtered to sterilize.
Embodiment 2
[0047] Embodiment 2 The present invention provides the preparation of culture medium
[0048] Mix 950mL LONZA X-VIVO 15 medium, 50mL human plasma, 4.0×10 5U of IL-2, 100 μg of IL-21, 100 μg of IL-15 and 100 μg of OKT-3 were mixed and filtered to sterilize.
Embodiment 3
[0049] Embodiment 3 The present invention provides the preparation of culture medium
[0050] Mix 850mL LONZA X-VIVO 15 medium, 150mL human plasma, 2.0×10 5 U of IL-2, 40 μg of IL-21, 60 μg of IL-15 and 40 μg of OKT-3 were mixed and filtered to sterilize.
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The invention relates to the cell culture technical field, and particularly relates to a natural killer cell culture medium and a natural killer cell amplification culture method. The invention provides the culture medium used for amplification culture of natural killer cells and containing a serum-free culture medium, human plasma, IL-2, IL-21, IL-15 and OKT-3. The culture medium provided by the invention is used for amplification culture of the natural killer cells, can avoid risks caused by exogenous serum, has high amplification efficiency and allows the obtained natural killer cells to have high purity. With adopting the method for amplification of the natural killer cells, amplification of the natural killer cells can be maintained at a logarithmic phase for a longer period of time. Moreover, the cultured natural killer cells have good killing activity on tumor cells.
Description
technical field [0001] The invention relates to the technical field of cell culture, in particular to a medium for natural killer cells and a method for expanding and culturing natural killer cells. Background technique [0002] Natural killer cells (NK), also known as NK cells, are considered to be the body’s first line of defense against infection and tumors. Associated with, and in some cases involved in, the development of hypersensitivity reactions and autoimmune diseases. NK cells are rich in cytoplasm and contain larger azurophilic granules, and the content of granules is positively correlated with the killing activity of NK cells. The killing effect of NK cells occurs early after acting on the target cells, and the killing effect can be seen in 1 hour in vitro and 4 hours in vivo. The target cells of NK cells mainly include certain tumor cells (including some cell lines), virus-infected cells, some self-tissue cells (such as blood cells), parasites, etc. Therefore,...
Claims
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Inventor 葛啸虎陈海佳王一飞应杰王小燕罗二梅
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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