An optimized polyvalent antigen gene sequence of enterotoxigenic Escherichia coli and its application in preventing diarrhea in weaned piglets

A technology of Escherichia coli and multivalent antigens, which is applied in the direction of bacterial antigen components, gene therapy, and medical preparations containing active ingredients, etc. Antibody interference and other issues, to achieve a comprehensive protective response, synergistically enhance the immune response, and improve the immune effect
CN104593397BInactive Publication Date: 2018-11-20NORTHEAST AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NORTHEAST AGRICULTURAL UNIVERSITY
Publication Date
2018-11-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an optimized enterotoxigenic escherichia coli (ETEC)-producing polyvalent antigen gene sequence and the application thereof in preventing weaned piglet diarrhea, and belongs to the technical field of biology. The polyvalent antigen gene sequence is synthesized by a large fragment according to the preference of the lactococcus lactis codon, and the synthesized polyvalent antigen gene sequence contains six antigen genes, namely common ETEC dominant serotype F4<+> primary structure protein FaeG causing the weaned piglet diarrhea, the receptor binding domain RBD of F18<+>, and toxins Stx2e and STa mutant, LTB subunit and STb, and molecular peptides CO1 and YadA31 genes targeting the M cells and the intestinal cells, respectively; the genes are connected by GGGGS. The genes are applicable to constructing a lactic acid bacterium living-vector vaccine and remarkably improving the secretory expression quantity of the target protein, and have excellent immunogenicity and protection effect; the genes also are suitable for high-efficiency expression in the escherichia coli; experiments prove that the inclusion body of the genes has excellent immunocompetence and can be taken as the vaccine for preventing the weaned piglets from F4<+> and F18<+> ETEC infection.
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Description

technical field

[0001] The invention relates to a synthetic codon-optimized polyvalent antigen gene sequence of enterotoxigenic Escherichia coli and an application thereof, belonging to the field of biotechnology. Background technique

[0002] Diarrhea in weaned piglets is a long-term problem that plagues pig farms and farmers. It not only brings direct death losses and huge medical expenses, but also indirectly affects the growth rate and breeding rate of rehabilitation pigs in the next stage. The damage is quite serious, and it has become a Important constraints to improving the economic performance of the swine industry worldwide. Enterotoxigenic Esckrichia coli (ETEC) is the main pathogen and an important cause of diarrhea in weaned piglets; among them, F4 + 、F18 + ETEC has a clear dominant position and therefore controls F4 + 、F18 + ETEC infection has become a key link in the prevention and treatment of diarrhea in weaned piglets. It has been reported that most of ...

Claims

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