Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Methods of detecting diseases or conditions

A technology of pathological conditions and diseases, applied in the field of identifying markers of diseases or pathological conditions, can solve the problem of important information loss and other problems

Inactive Publication Date: 2015-05-06
哈里斯泰利
View PDF201 Cites 30 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method can enrich the signal to be detected, it also results in the loss of potentially important information

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Methods of detecting diseases or conditions
  • Methods of detecting diseases or conditions
  • Methods of detecting diseases or conditions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0405] Representative method for separating phagocytic cells with a DNA content of 2n from non-phagocytic cells and analyzing expression profiles 1

[0406] 1. Separate blood samples into plasma and buffy coat including WBC samples. Plates were coated with avidin to accept WBC samples.

[0407] 2. Add biotinylated antibody to non-phagocytic blood cells (such as T cells) in the wells, incubate at RT for 30 minutes, and wash the wells.

[0408] 3. Add magnetic beads.

[0409] 4. Add WBC blood sample.

[0410] 5. Incubate at 37°C (30 minutes - 1 hour).

[0411] 6. After the beads have been phagocytized by the phagocytes and the avidin-biotin-antibody has bound to the non-phagocytic cells, place the plate on a magnet and wash (phagocytes internalizing the magnetic beads and non-phagocytic cells bound to the antibody will be retained; all other cells will be washed away).

[0412] 7. Remove magnet and collect phagocytic cells and separate into phagocytic cells with DNA equal t...

Embodiment 2

[0414] Representative method for separating phagocytic cells from non-phagocytic cells II

[0415] 1. Separate blood samples into plasma and buffy coat including WBC samples.

[0416] 2. Cytospin WBC onto glass slides.

[0417] 3. Fix cells in acetone / methanol (-20°C for 5 minutes).

[0418] 4. Stain with hematoxylin and eosin and anti-T cell antibody.

[0419] 5. Isolation of T cells (non-phagocytic) and macrophages (phagocytic) using laser capture microscopy (LCM). Divide into phagocytic cells with DNA equal to 2n and DNA greater than 2n. Non-phagocytic and phagocytic cells with DNA equal to 2n are called cells with DNA equal to 2n.

Embodiment 3

[0421] Representative method for separating phagocytic cells from non-phagocytic cells III

[0422] 1. Separate plasma from whole blood.

[0423] 2. Isolation of non-phagocytic cells (such as T cells) and phagocytic cells (such as neutrophils and / or macrophages and / or monocytes) from whole blood using magnetic antibody-conjugated beads. Divide into phagocytic cells with DNA equal to 2n and DNA greater than 2n. Non-phagocytic and phagocytic cells with DNA equal to 2n are called cells with DNA equal to 2n.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

This invention provides methods of using a sample with multiple analytical components in the diagnosis, prognosis, or monitoring of diseases or conditions. The invention also provides methods of identifying markers of diseases or conditions.

Description

[0001] priority information [0002] This application claims priority to US Provisional Application 61 / 660,427, filed June 15, 2012. The content and disclosure of said application is incorporated herein by reference in its entirety. field of invention [0003] The present invention generally relates to methods of using a combination of two or more different components selected from acellular body fluids, phagocytes, circulating vesicles and circulating diseased cells in the diagnosis, prognosis or monitoring of a disease or condition. The invention also relates to methods of using the combination to identify markers of a disease or condition. Background of the invention [0004] Leukocytes begin as multipotent hematopoietic stem cells in the bone marrow and progress along the myeloid lineage (monocytes, macrophages, neutrophils, eosinophils, and basophils) or the lymphoid lineage (T and B lymphocytes and natural killer cells) development. The primary function of cells of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/53C40B30/04
CPCC12Q1/6883G01N33/5023G01N33/56966C12Q2600/158C12Q2600/136C12Q2600/118G01N33/5008G01N33/68G01N33/92C12Q2600/156C12Q2600/16G01N33/6803G01N2570/00
Inventor 哈里·斯泰利
Owner 哈里斯泰利
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com