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Oak leaf extract, as well as preparation method and application thereof

A technology of tussah leaves and extracts is applied in the field of preparation of tussah leaves extracts, and achieves the effects of improving utilization rate, less equipment investment, and prolonging shelf life

Active Publication Date: 2015-05-13
辽宁省农业科学院大连生物技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports on the use of eucalyptus leaves to extract antibacterial active ingredients and use them for food preservation, external use to treat skin and mucous membrane infectious diseases caused by bacteria, fruit and vegetable preservation and anti-corrosion, and external use of cosmetics for anti-inflammation.

Method used

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  • Oak leaf extract, as well as preparation method and application thereof
  • Oak leaf extract, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A preparation method of Quercus eucalyptus leaf extract, said preparation method comprising the steps of:

[0026] ①Wash the oak leaves, dry them in the shade at 25°C, crush them to 40 meshes, mix them with deionized water at a material-to-liquid ratio of 1g / 15mL, leaching at 20°C for 12 hours, leaching 3 times, combining, filtering, and centrifuging at 3000rpm for 10 minutes to obtain the leaching solution, the leaching solution was refluxed at 65°C in a water bath and concentrated to 6.67% of the volume of the leaching solution to obtain a 1 g / mL concentrated solution;

[0027] ② Dilute the 1g / mL concentrated solution obtained in step ① to 1g / 10mL with deionized water, filter, and use the ultrafiltration membrane device with a molecular weight cut-off of 5000Dr or less to filter the filtrate at 0.05-0.08MPa to collect substances with a molecular weight below 5000Dr , and then use the ultrafiltration membrane device with a molecular weight cut-off of 500Dr or more to f...

Embodiment 2

[0029] A preparation method of Quercus eucalyptus leaf extract, the difference from Example 1 is: wash the eucalyptus leaves, dry them in the shade at 25°C, pulverize them to 40 meshes, mix them with 65% ethanol according to the ratio of material to liquid at 1g / 15mL, soak them at 20°C Take 12 hours, leaching 3 times, combine, filter, centrifuge at 3000rpm for 10min to obtain the leaching solution, reflux the leaching solution at 60°C to 6.67% of the volume of the leaching solution to obtain the first concentrated solution, and concentrate to the first time Add 10 times the volume of deionized water to the solution, reflux at 60°C and concentrate to the volume of the first concentrated solution to obtain a 1 g / mL concentrated solution.

Embodiment 3

[0031] A preparation method of Quercus eucalyptus leaf extract, the difference from Example 1 is: wash the eucalyptus leaves, dry in the shade at 25°C, pulverize them to 40 meshes, mix with 75% ethanol according to the ratio of material to liquid at 1g / 15mL, soak at 20°C Take 12 hours, leaching 3 times, combine, filter, centrifuge at 3000rpm for 10min to obtain the leaching solution, reflux the leaching solution at 60°C to 6.67% of the volume of the leaching solution to obtain the first concentrated solution, and concentrate to the first time Add 10 times the volume of deionized water to the solution, reflux at 60°C and concentrate to the volume of the first concentrated solution to obtain a 1 g / mL concentrated solution.

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Abstract

The invention relates to an oak leaf extract as well as a preparation method and application thereof and belongs to the field of extraction technologies. The preparation method comprises the following steps: (1) leaching an oak leaf to form a leaching solution, condensing the leaching solution, and (2) ultra-filtering the product obtained in Step (1) to form the oak leaf extract with molecular weight of 500-5000Dr. The oak leaf extract has the benefit that the prepared oak leaf extract has a bacteriostatic action.

Description

technical field [0001] The invention relates to an oak leaf extract and its preparation method and application, belonging to the field of extraction technology. Background technique [0002] Oak tree (Querus mongolica Fisch) is a deciduous tree of the family Fagaceae, the genus Quercus. There are species of Quercus mongolica, Quercus liaotungensis, Quercus mongolica, and oak tree. The leaf margins are serrated and rarely entire. The catkins of the male flowers are drooping, and the female flowers are solitary in the involucre. The nut is solitary, the inner wall of the pericarp is glabrous, and the rudimentary ovule is located outside the base of the seed. The trunk is peculiar and vigorous, the shape of the tree is beautiful and varied, the branches and leaves are luxuriant, it is resistant to pruning, and it is easy to shape. There are more than 300 species in the world, more than 60 species in China, and the oak forest area is about 100 million mu. It has the character...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61K8/97A23L3/3472A23B7/153A61P31/04A61K127/00
Inventor 王林美岳冬梅夏兴宏李树英
Owner 辽宁省农业科学院大连生物技术研究所
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