peg-plga encapsulated hemoglobin

A hemoglobin and encapsulation technology, applied in the field of blood substitutes, can solve problems such as safety and in vivo effectiveness that have not been reported, achieve good oxygen supply effect, solve vasoconstriction, and high stability.

Active Publication Date: 2018-07-10
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 1997, Chang et al used PLA, PLGA and polyisobutycyanoacrylate mixture as a packaging material to wrap hemoglobin, with particle size distribution from 70nm to 1110nm, but its safety and in vivo effectiveness have not been reported (Chang TMS, Yu WP.Biodegradable polymer membrane containing hemoglobin for oxygen carrier.US Patent 5670173,1997.)

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0066] Embodiment 1, preparation PEG-PLGA encapsulated hemoglobin

[0067] The preparation of PEG-PLGA wrapped hemoglobin of the present invention comprises the following steps:

[0068] 1) Dissolve PEG-PLGA in ethyl acetate to make the concentration 20mg / mL (10-30mg / mL is acceptable), then mix 2mL PEG-PLGA solution with 0.4mL 120mg / mL (60-240mg / mL is acceptable) The aqueous solution of hemoglobin is mixed according to the mass ratio of 5:6 (5:4-5:12 is acceptable), and the ultrasonic cell disruptor is used to sonicate in an ice water bath at 80W (80-100W is acceptable) for 3min (3-5min is acceptable) , forming colostrum;

[0069] 2) Mix 10mL of surfactant aqueous solution, in this example, 7mL of 2wt% PVA aqueous solution and 3mL of 2wt% F68 aqueous solution (volume ratio 7:3, volume ratio 1:1-9:1 can also be used, and a single PVA Aqueous solution), add 10mL of the mixture of PVA aqueous solution and F68 aqueous solution to the colostrum obtained in step 1), mix, and use a...

Embodiment 2

[0071] Embodiment 2, the physicochemical property detection of PEG-PLGA encapsulated hemoglobin

[0072]The physical and chemical properties of the PEG-PLGA coated hemoglobin particles prepared in Example 1 were detected as follows:

[0073] 1. Observation of particles by transmission electron microscope

[0074] The particle morphology was observed with a transmission electron microscope (model JEM-200CX, purchased from JEOL Ltd.).

[0075] The result is as figure 1 As shown, the prepared PEG-PLGA-encapsulated hemoglobin is spherical particles, and these particles are uniformly dispersed without agglomeration. It is preliminarily confirmed that the prepared PEG-PLGA-encapsulated hemoglobin is a nanoparticle colloidal dispersion system with concentrated particle size distribution. Each PEG-PLGA coated hemoglobin particle shape and figure 1 The result was no difference.

[0076] 2. Determination of the particle size distribution of nanoparticles by dynamic light scattering ...

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Abstract

The invention discloses a hemoglobin oxygen carrier, PEG-PLGA, to encapsulate hemoglobin. The PEG-PLGA encapsulated hemoglobin of the present invention is the encapsulated hemoglobin obtained by encapsulating the hemoglobin with polyethylene glycol-polylactic acid glycolic acid block copolymer (PEG-PLGA) nanoparticles, the particle size distribution is uniform, the dispersibility is good, and the oxygen It has high affinity and good oxygen supply effect, and can be used in systemic or local hypoxia, or in systems that require oxygen supply in vitro. The hemoglobin oxygen carrier-PEG-PLGA encapsulated hemoglobin of the present invention will play an important role in the medical field as a blood substitute, and has broad application prospects.

Description

technical field [0001] The invention belongs to blood substitutes in the medical field, in particular to a new type of hemoglobin oxygen carrier-PEG-PLGA wrapped hemoglobin and its application. Background technique [0002] Blood transfusion plays a pivotal role in surgical medicine and war wound treatment, and more and more people pay attention to the source and safety of blood supply. Because blood substitutes have the advantages of "good versatility, high safety, and long shelf life", the research and development of blood substitutes is of great significance and has attracted much attention. Among them, hemoglobin-based oxygen carriers (HBOCs) are research hotspots in the field of blood substitutes. kind. Among them, the cell-like structure wrapped hemoglobin is to wrap the outside of the hemoglobin with a membrane to make it closer to the actual structure of red blood cells. Toxicity, increased circulation time in the body. According to different materials, hemoglobi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K47/34A61K38/42A61P7/08A61P39/00A61P9/10A61P35/00
Inventor 周虹赵莲王瑛卢明子陈赣王权尤国兴赵敬湘王波尹玉静
Owner ACADEMY OF MILITARY MEDICAL SCI
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