The invention relates to the field of micro-fluidic
chip molecular diagnosis, and in particular to a molecular diagnosis micro-fluidic
chip, a molecular diagnosis micro-fluidic
chip system and applications of the molecular diagnosis micro-fluidic chip and the molecular diagnosis micro-fluidic chip
system. The chip comprises a sample injection unit (1) which is used for receiving to-be-detected samples, at least one
liquid storage unit (2) for storing reaction reagents, a
cell lysis unit (3) for conducting
cell lysis and a
nucleic acid amplification unit (4) for conducting
nucleic acid amplification, wherein the
cell lysis unit comprises a slit (31) as well as a first compartment (32) and a second compartment (33) which are separated by virtue of the slit; at least one compartment is configured with
grinding micro-beads; the first compartment communicates with the sample injection unit; and the second compartment communicates with the at least one
liquid storage unit, so as to receive acell lysis solution. The chip
system is composed of three
layers, namely an upper layer, a middle layer and a lower layer, wherein the chip is arranged on the middle layer; the upper and lower layersare used for covering and sealing the middle layer; the upper layer is provided with a sample injection hole which is connected to the sample injection unit, and the sample injection hole correspondsto an avoiding hole of the
liquid storage unit. The chip is simple in detection process, high in sensitivity and strong in
repeatability, and reaction samples can be quantified.