A centrifugal microfluidic chip and capillary gel electrophoresis device for capillary electrophoresis
A capillary electrophoresis and microfluidic chip technology, which is used in measuring devices, laboratory containers, and material analysis by electromagnetic means, can solve problems such as high cost and low sensitivity, and achieve improved sensitivity, broad market prospects, The effect of increasing processing throughput
Inactive Publication Date: 2016-06-08
方雪萍 +1
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Problems solved by technology
[0003] In order to solve the problems of high cost and low sensitivity of existing silicon capillary microfluidic chips, the present invention provides a centrifugal microfluidic chip for capillary electrophoresis
Method used
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Embodiment 1
[0060] In the centrifugal microfluidic chip provided by the present invention, the slide is circular, with a thickness of 1.5 mm, an outer diameter of 120 mm, and an inner diameter of 15 mm. The capillary channel is approximately 300 mm long, 50 microns wide, and 500 microns deep.
[0061] The chip cover is made of the same material as the slide, with a thickness of 1.0 mm, a diameter of 120 mm, and an inner diameter of 15 mm. The chip cover is glued to the slide.
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Abstract
The invention belongs to the fields of medicine analysis and detection, particularly relates to a micro-fluidic chip for capillary electrophoresis. The invention provides a centrifugal micro-fluidic chip for capillary electrophoresis to solve the problems that an existing silicon capillary micro-fluidic chip is relatively high in cost and relatively low in sensitivity. The chip comprises a slide glass and a chip cover, wherein capillary electrophoresis channels are formed in the slide glass, and are distributed along the radial direction of the chip; cooling liquid channels are arranged nearby the capillary electrophoresis channels; the initial end of each capillary electrophoresis channel is communicated with a sample pool and a buffer liquid pool; the tail end of each capillary electrophoresis channel is communicated with a waste liquid pool; a cathode inserting hole is formed in the initial end of each capillary electrophoresis channel; an anode inserting hole is formed in each tail end; a gravity control valve is arranged at the tail end of each capillary electrophoresis channel; and cooling liquid connectors are formed at two ends of the cooling liquid channels. The centrifugal micro-fluidic chip is low in cost, high in sensitivity and high in processing throughput.
Description
technical field [0001] The invention belongs to the field of medical analysis and detection, in particular to a microfluidic chip for capillary electrophoresis. Background technique [0002] At present, the commonly used microfluidic chip for capillary electrophoresis is a silicon capillary microfluidic chip. The cross section of the silicon capillary is usually circular. In the case of the same cross section, the optical path is shorter than the rectangular cross section, so the sensitivity is relatively high. Low. At the same time, the cost of the silicon capillary microfluidic chip is relatively high. Contents of the invention [0003] In order to solve the problems of high cost and low sensitivity of the existing silicon capillary microfluidic chip, the invention provides a centrifugal microfluidic chip for capillary electrophoresis. The centrifugal microfluidic chip has low cost, high sensitivity and high processing throughput. [0004] In order to achieve the abov...
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IPC IPC(8): B01L3/00G01N27/447
Inventor 方雪萍陈松明
Owner 方雪萍