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A method of enzymatically preparing oil

An oil and lipase technology, applied in the fields of edible oil/fat, food science, application, etc., can solve the problems of increased content of chloropropanol esters and glycidyl esters, adverse effects, etc., to improve product quality and safety. High, good quality results

Active Publication Date: 2020-10-30
WILMAR SHANGHAI BIOTECH RES & DEV CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, transesterification or esterification results in elevated levels of chloropropanol esters and glycidyl esters, which are internationally recognized carcinogenic pollutants or probable hereditary carcinogens
Oil products prepared by enzymatic transesterification or enzymatic esterification will have adverse effects on the human body if they pollute the environment or daily necessities or are eaten by humans

Method used

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  • A method of enzymatically preparing oil
  • A method of enzymatically preparing oil
  • A method of enzymatically preparing oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1 The influence of no pretreatment enzyme column on chloropropanol ester and glycidyl ester in oil

[0054] A mixture of rapeseed oil and medium-chain triglycerides (C8 / C10=50 / 50, w / w) with a molar ratio of 0.55:1 was passed through the packed column with lipase Lipozyme RM IM at a flow rate of 2 times the enzyme amount per hour, The enzymatic transesterification reaction was carried out at 70°C. Then the reaction product was heated at 180°C under a vacuum of 10 -3 mbar, internal cooling temperature of 30°C for molecular distillation separation and purification, collect long-chain triglycerides in the heavy phase, and then pass nitrogen into the heavy phase (as a stirring and deodorizing medium), at a vacuum degree of about 10-20mbar, 200°C Deodorize at temperature for 1 hour, then remove the heating mantle, break the vacuum below 50°C and stop the nitrogen. The grease 1 was obtained by filtering with a 5 micron filter bag.

[0055] The soap content of t...

Embodiment 2

[0058] Example 2 Effect of pretreatment enzyme column on chloropropanol ester and glycidyl ester in oil

[0059] The column to which 200 g of Lipozyme RM IM enzyme was added was eluted with 600 g of a mixture of rapeseed oil and medium chain triglycerides (C8 / C10 = 50 / 50, w / w) (molar ratio 0.55:1). Then the mixture of rapeseed oil and medium-chain triglycerides (C8 / C10=50 / 50, w / w) with a molar ratio of 0.55:1 was passed through a lipase-packed column at a flow rate of 2 times the enzyme amount per hour at 70°C. Enzymatic transesterification reaction. Afterwards, the reaction product was heated at 180°C under a vacuum of 10 -3 Mbar, internal cooling temperature 30 ℃ molecular distillation separation and purification. Then pass nitrogen gas into the heavy phase (as a stirring and deodorizing medium), and deodorize at a vacuum degree of about 10-20 mbar and a temperature of 200 ° C for 1 hour. Then remove the heating mantle, break the vacuum below 50°C and stop the nitrogen,...

Embodiment 3

[0064] Embodiment 3 The influence of molecular distillation temperature on chloropropanol ester and glycidyl ester in oil

[0065]The column to which 200 g of Lipozyme TL IM enzyme was added was eluted with 1000 g of a mixture of sunflower oil and medium-chain fatty acids (caprylic acid / capric acid = 40 / 60, w / w) (molar ratio 0.75:1). Then the mixture of sunflower oil and medium-chain fatty acid (caprylic acid / capric acid=40 / 60, w / w) with a molar ratio of 0.75:1 was passed through a lipase-packed column at a flow rate of 2 times the enzyme amount per hour at 50°C. Enzymatic transesterification reaction. Afterwards, the reaction product was vacuumed at 10 -3 mbar, molecular distillation was carried out at 160°C, 180°C, 200°C, 220°C, and 240°C at an internal cooling temperature of 30°C. Afterwards, use nitrogen as a stirring and deodorizing medium, and deodorize for 1.5 hours at a vacuum degree of about 10-20 mBar and a temperature of 200°C. Finally, filter with a 5 micron f...

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Abstract

The invention provides a method for preparation of grease by enzymatic method, and the method is as follows: 1, pretreating lipase with reaction raw materials; 2, performing enzymatic transesterification reaction and enzymatic esterification on the reaction raw materials in the presence of the pretreated lipase; and 3, separating a required grease product from the reaction products, and deodorizing the grease product. The method omits the destaining step in the traditional process, and reduces the content of chloropropyl alcohol ester and glycidyl ester, and the soap content is low.

Description

technical field [0001] The invention relates to the preparation, purification and refining of enzymatic fats and oils. Specifically, the present invention controls impurities such as chloropropanol esters and glycidyl esters in enzymatic fats and oils through pretreatment of lipase, separation of reaction products and deodorization. Background technique [0002] In order to obtain specific oils that do not exist or are rare in nature, transesterification or esterification can be used. If a chemical transesterification method or a chemical esterification method is used, the positioning and distribution of a certain fatty acid on the glycerol molecule cannot be controlled, so the purpose is often not achieved. If enzymatic transesterification or enzymatic esterification is used, because lipase has position selectivity, it can act on the catalytic product purposefully, so as to carry out molecular design from the structure and prepare oil products with high added value. Moreo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23D9/02
Inventor 周盛敏李磊张余权
Owner WILMAR SHANGHAI BIOTECH RES & DEV CENT