Profile model-based geometric correction method for optical projection tomography imaging system

Abstract

The invention discloses a profile model-based geometric correction method for an optical projection tomography imaging system. The profile model-based geometric correction method comprises the following steps: carrying out data collection on a profile model to obtain two-dimensional projection data; carrying out three-dimensional reconstruction on the two-dimensional projection data to obtain three-dimensional reconstruction data, wherein the three-dimensional reconstruction data comprise horizontal offset information and rotation information of a sample rotating axis; acquiring horizontal offset and rotation angle, corresponding to the horizontal offset information and rotation information of the sample rotating axis, of the rotating axis according to the horizontal offset information and the rotation information of the sample rotating axis; and carrying out geometric correction on horizontal offset and rotation offset, corresponding to the horizontal offset and the rotation angle of the rotating axis, through the horizontal offset and the rotation angle of the rotating axis. According to the profile model-based geometric correction method, the spatial resolution rate of the optical projection tomography imaging system can be improved; and artifacts of images are reduced.

Description

technical field [0001] The invention relates to image processing technology, in particular to a geometric correction method of a phantom-based optical projection tomography system. Background technique [0002] Optical Projection Tomography (OPT) can realize structural and molecular specific imaging of biological samples at the scale of 1-10 mm. It has many advantages such as high resolution, integrated structure and function, no radiation, and low cost. It can be used in Carry out qualitative and quantitative research on the cellular level of living organisms on a small scale to achieve real-time, non-invasive, dynamic, and in vivo imaging of organisms. At present, OPT technology has been applied to the imaging of small animal embryos and complete organs such as mice, and the imaging of model animals such as Drosophila. Imaging of model animals such as mice and Drosophila is of great significance for the study of gene expression, protein interaction, and preclinical drug d...

AI Technical Summary

Problems solved by technology

However, if during the imaging process, the rotation axis of the sample deviates from the center line of the acquired image (that is, the deviation of the rotation axis of the turntable from the center line of the camera), and the deviation is not corrected before reconstruction, even if the deviation is only a few pixels, can also cause artifacts in the reconstructed image

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