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A Serum Biochemical Marker for Assisted Screening of Low-fat Broilers and Its Application

A biochemical index and serum technology, applied in the field of avian genetics and breeding, can solve the problems of long time and poor selection effect, and achieve the effect of high heritability and stable inheritance

Active Publication Date: 2016-09-14
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This selection method takes a long time, and the selection effect is not very good.

Method used

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  • A Serum Biochemical Marker for Assisted Screening of Low-fat Broilers and Its Application
  • A Serum Biochemical Marker for Assisted Screening of Low-fat Broilers and Its Application
  • A Serum Biochemical Marker for Assisted Screening of Low-fat Broilers and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Experimental materials

[0026] 1. Experimental Animals and Determination of Traits

[0027] The 16th, 17th and 18th generation cocks of broiler high-fat and low-fat two-way selection lines bred by Northeast Agricultural University were used as the experimental groups. At the age of 7 weeks, after fasting for 12 hours, the live weight of broilers was measured before slaughter, and the abdominal fat weight was measured after slaughter, and the abdominal fat rate was calculated by dividing by the live weight at 7 weeks old. Carotid artery blood was collected and placed in a 5ml centrifuge tube (without adding anticoagulant), the blood sample was centrifuged at 3000r / min for 15min to obtain serum, put into an EP tube, and stored in a -20°C refrigerator for later use.

[0028] 2. Drugs and enzymes

[0029] Triglyceride detection kit; total cholesterol detection kit; high-density lipoprotein cholesterol detection kit; low-density lipoprotein cholesterol detection kit; t...

Embodiment 2

[0045] Example 2 Comparison of Chicken Serum Biochemical Indexes Between High and Low Fat Two-way Selection Line Broilers

[0046] The 16th, 17th and 18th generation broiler chickens of the high-fat and low-fat two-way selection line selected by Northeast Agricultural University were used as experimental materials to compare the body fat traits of high-fat and low-fat broiler chickens in each generation analyze. The results showed that in the three generations, the abdominal fat weight and abdominal fat rate of high-fat broiler chickens were significantly higher than those of low-fat broiler chickens (P<0.01) (Table 1).

[0047] Table 1 Comparison of body fat traits between the sixteenth, seventeenth and eighteenth generations of high-fat and low-fat broiler chickens

[0048]

[0049] Note: A and B are in the same row, indicating that there is a significant difference between high-fat and low-fat broilers (P<0.01)

[0050]Using the sixteenth, seventeenth and eighteenth ge...

Embodiment 3

[0061] The genetic parameter estimation of embodiment 3 serum indexes

[0062] The serum biochemical indicators of the three generations obtained in Example 2 are put together to estimate genetic parameters, and the results show that serum albumin / globulin (h 2 =0.89) and high-density lipoprotein cholesterol / low-density lipoprotein cholesterol (h 2 =0.86) the highest heritability, followed by serum total bile acid, low-density lipoprotein cholesterol and albumin (0.582 2 2 <0.18) (Table 5).

[0063] Serum HDL-C / LDL-C, HDL-C, and glucose concentrations were positively associated with higher abdominal fat traits (r g )(0.30g g p )(-0.29p <0.28) (Table 5).

[0064] Table 5 Heritability of serum biochemical indicators, genetic and epigenetic correlations between serum biochemical indicators and body fat traits

[0065]

[0066] Note: HDL-C high-density lipoprotein cholesterol; LDL-C low-density lipoprotein cholesterol.

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Abstract

The invention discloses a serum biochemical marker for auxiliary screening of low-fat broiler chicken and application of a serum biochemical marker, and belongs to the technical field of poultry genetic breeding. The serum biochemical marker disclosed by the invention is the level of HDL-C / LDL-C in chicken serum. The marker has significant difference in the low-fat broiler chicken and high-fat broiler chicken, also has high genetic correlation coefficient and hereditary capacity, and can be applied to identification of abdominal fat amount of the broiler chicken and breeding of the low-fat broiler chicken. In addition, the invention also provides the application and a screening method of the serum biochemical marker.

Description

technical field [0001] The invention relates to a serum biochemical marker for auxiliary screening of low-fat broilers and its application, belonging to the technical field of poultry genetics and breeding. Background technique [0002] Over the past half century, broiler breeders have devoted themselves to the selection of broiler growth rate and feed conversion efficiency, and have made remarkable genetic progress. However, the rapid increase in broiler growth rate has brought about negative factors such as abdominal fat deposition and metabolic syndrome. Excessive abdominal fat deposition has many disadvantages. It not only affects feed efficiency, but also affects broiler carcass quality. Therefore, controlling the excessive accumulation of fat in the chicken body and further improving the feed conversion efficiency and carcass quality of broilers are major issues urgently needed to be studied and solved in our country. Breeding low-fat broiler mating lines is one of t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/92
CPCG01N33/92
Inventor 李辉张慧董佳强
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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