Dendrobium officinale extract as well as preparation method and application thereof
A technology of Dendrobium officinale and Dendrobium officinale polysaccharide, which is applied in application, food preparation, food science, etc., can solve the problems of insufficient utilization of active ingredients, unclear material basis, single active ingredient, etc. Large effect range and short extraction time
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Embodiment 1
[0026] Embodiment 1 Content determination method
[0027] (1) Determination of polysaccharide content
[0028] Preparation of reference substance solution Take an appropriate amount of anhydrous glucose reference substance, accurately weigh it, and add water to make a solution containing 90 μg per 1ml, that is.
[0029] Preparation of standard curve Precisely measure 0.2ml, 0.4ml, 0.6ml, 0.8ml and 1.0ml of the reference solution, put them in 10ml test tubes with stoppers, add water to make up to 1.0ml each, and accurately add 1ml of 5% phenol solution (for clinical use). Preparation), shake well, then add 5 ml of sulfuric acid precisely, shake well, heat in a boiling water bath for 20 minutes, take out, cool in an ice bath for 5 minutes, take the corresponding reagent as a blank, according to UV-Vis spectrophotometry, at 488nm Measure the absorbance at the wavelength, take the absorbance as the ordinate and the concentration as the abscissa, and draw a standard curve.
[003...
Embodiment 2
[0046] Example 2 Preparation of Dendrobium officinale extract
[0047] (1) Degreasing: take 500 g of decoction pieces of Dendrobium officinale, add 5 times the amount of petroleum ether to soak for 0.5 h, and then reflux for 2 times, 2 h each time, filter, and evaporate the organic solvent to obtain medicinal residues;
[0048] (2) Flash extraction: take the above-mentioned medicinal residues, add 5 times the amount of 30% ethanol solution to soak for 0.5 hours, and then perform flash extraction. The flash extraction time is 1 min, and the flash extraction speed is 3500 rpm. Until there is no alcohol smell, each ml of the concentrate is equivalent to 1.0g of crude drug;
[0049] (3) Deproteinization: Take the above-mentioned concentrate, add Sevage reagent 1 / 5 times the volume of the concentrate, shake vigorously for 10 minutes, and after standing for 10 minutes, centrifuge at 4000 r / min for 10 minutes, the solution is divided into three layers, the upper layer solution is col...
Embodiment 3
[0051] Example 3 Preparation of Dendrobium officinale extract
[0052] (1) Degreasing: take 500 g of decoction pieces of Dendrobium officinale, add 8 times the amount of n-hexane and soak for 1 hour, then reflux once for 2 hours each time, filter, and evaporate the organic solvent to obtain medicinal residues;
[0053] (2) Flash extraction: take the above-mentioned medicinal residues, add 20 times the amount of 5% ethanol solution to soak for 1 hour, and then carry out flash extraction. The flash extraction time is 3 minutes, and the flash extraction speed is 4500 rpm. No alcohol smell, each ml of the concentrate is equivalent to 0.5g of crude drug;
[0054] (3) Deproteinization: Take the above concentrate, add Sevage reagent 1 / 4 times the volume of the concentrate, shake vigorously for 10 minutes, stand for 10 minutes, centrifuge at 5000 r / min for 10 minutes, the solution is divided into three layers, collect the upper layer solution, the upper layer The solution repeats the...
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