Dendrobium officinale extract as well as preparation method and application thereof

A technology of Dendrobium officinale and Dendrobium officinale polysaccharide, which is applied in application, food preparation, food science, etc., can solve the problems of insufficient utilization of active ingredients, unclear material basis, single active ingredient, etc. Large effect range and short extraction time

Active Publication Date: 2015-07-01
ZHEJIANG JIGONGYUAN PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of the preparation methods of Dendrobium officinale extracts reported in the literature focus on polysaccharides, and the active ingredients are single. The extraction methods mainly include heating extraction, enzymatic hydrolysis, ultrasonic-assisted extraction, microwave-assisted extraction, etc., and water is used as the extraction method. Solvent, there are disadvantages such

Method used

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  • Dendrobium officinale extract as well as preparation method and application thereof
  • Dendrobium officinale extract as well as preparation method and application thereof
  • Dendrobium officinale extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 Content determination method

[0027] (1) Determination of polysaccharide content

[0028] Preparation of reference substance solution Take an appropriate amount of anhydrous glucose reference substance, accurately weigh it, and add water to make a solution containing 90 μg per 1ml, that is.

[0029] Preparation of standard curve Precisely measure 0.2ml, 0.4ml, 0.6ml, 0.8ml and 1.0ml of the reference solution, put them in 10ml test tubes with stoppers, add water to make up to 1.0ml each, and accurately add 1ml of 5% phenol solution (for clinical use). Preparation), shake well, then add 5 ml of sulfuric acid precisely, shake well, heat in a boiling water bath for 20 minutes, take out, cool in an ice bath for 5 minutes, take the corresponding reagent as a blank, according to UV-Vis spectrophotometry, at 488nm Measure the absorbance at the wavelength, take the absorbance as the ordinate and the concentration as the abscissa, and draw a standard curve.

[003...

Embodiment 2

[0046] Example 2 Preparation of Dendrobium officinale extract

[0047] (1) Degreasing: take 500 g of decoction pieces of Dendrobium officinale, add 5 times the amount of petroleum ether to soak for 0.5 h, and then reflux for 2 times, 2 h each time, filter, and evaporate the organic solvent to obtain medicinal residues;

[0048] (2) Flash extraction: take the above-mentioned medicinal residues, add 5 times the amount of 30% ethanol solution to soak for 0.5 hours, and then perform flash extraction. The flash extraction time is 1 min, and the flash extraction speed is 3500 rpm. Until there is no alcohol smell, each ml of the concentrate is equivalent to 1.0g of crude drug;

[0049] (3) Deproteinization: Take the above-mentioned concentrate, add Sevage reagent 1 / 5 times the volume of the concentrate, shake vigorously for 10 minutes, and after standing for 10 minutes, centrifuge at 4000 r / min for 10 minutes, the solution is divided into three layers, the upper layer solution is col...

Embodiment 3

[0051] Example 3 Preparation of Dendrobium officinale extract

[0052] (1) Degreasing: take 500 g of decoction pieces of Dendrobium officinale, add 8 times the amount of n-hexane and soak for 1 hour, then reflux once for 2 hours each time, filter, and evaporate the organic solvent to obtain medicinal residues;

[0053] (2) Flash extraction: take the above-mentioned medicinal residues, add 20 times the amount of 5% ethanol solution to soak for 1 hour, and then carry out flash extraction. The flash extraction time is 3 minutes, and the flash extraction speed is 4500 rpm. No alcohol smell, each ml of the concentrate is equivalent to 0.5g of crude drug;

[0054] (3) Deproteinization: Take the above concentrate, add Sevage reagent 1 / 4 times the volume of the concentrate, shake vigorously for 10 minutes, stand for 10 minutes, centrifuge at 5000 r / min for 10 minutes, the solution is divided into three layers, collect the upper layer solution, the upper layer The solution repeats the...

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Abstract

The invention provides a dendrobium officinale extract. In the extract, the content of dendrobium officinale polysaccharides is 70.0-90.0%, and the content of general flavones is 0.5-1.0%. The dendrobium officinale extract is capable of being used for preparing medicines or health products for preventing and treating embolic cardiovascular and cerebrovascular diseases and renal diabetes. The invention further provides a preparation method of the extract. The preparation method specifically comprises the four steps of organic solvent degreasing, flash extraction after ethanol solution soaking, Sevage reagent deproteinization and active carbon decolouration. The preparation method is capable of protecting the dendrobium officinale effective ingredient not to be thermally broken and furthest effectively extracting the dendrobium officinale polysaccharides and the flavones simultaneously, low in solvent dosage, short in extraction time, and capable of improving product quality and reducing production cost.

Description

technical field [0001] The invention belongs to the field of medicine and health food, and in particular relates to a dendrobium officinale extract and a preparation method and application thereof. Background technique [0002] Dendrobium officinale Kimura.et Migo (Dendrobium officinale Kimura.et Migo) is a perennial herb of the Orchidaceae Dendrobium genus. Its main medicinal parts are fresh or dry stems. It is mainly distributed in Anhui, Zhejiang, Guangxi, Hunan, Yunnan, Guizhou and other places. Chinese herbal medicine. Dendrobium officinale contains dendrobium polysaccharides, flavonoids, proteins, 17 kinds of amino acids and inorganic elements, etc. "Shen Nong's Materia Medica" listed it as the top grade, and the Taoist health classic "Dao Zang" praised it as the first of the "Nine Chinese Immortal Herbs". The folks call Dendrobium officinale "life-saving fairy grass" and "inch golden grass". [0003] At present, most of the preparation methods of Dendrobium officin...

Claims

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Application Information

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IPC IPC(8): A61K36/8984A61K31/715A61P7/02A61P9/00A61P13/12A61P3/10A23L1/09
Inventor 王建方吴健印晓红陈琳虞利东朱明旺李立雷汤郑静曹晓铃
Owner ZHEJIANG JIGONGYUAN PHARMA
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