A plant tissue culture tank
A technology of plant tissue culture and culture tank, which is applied in the direction of tissue cell/virus culture device, bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, etc., which can solve the problem of uneven mixing, plant isolation Tissue damage, small shear force of bubble reactor, etc.
Inactive Publication Date: 2017-09-19
CHANGCHUN INST OF TECH
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- Abstract
- Description
- Claims
- Application Information
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Problems solved by technology
[0010] The purpose of the present invention is to solve the problem that the stirred reactor has a large shear force and is easy to cause damage to the isolated plant tissue, while the bubble reactor has a small shear force and uneven mixing, and provides a plant tissue culture tank
Method used
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Embodiment 1
[0028] See Figure 1-3 , a plant tissue culture tank, which includes: a culture tank 1, a tank external circulation device 2, a pneumatic circulation device 3, a compressed air nozzle 4, a gas distributor 5, and an exhaust device;
[0029] Tank external circulation device 2 comprises: adding liquid pipe valve 21, adding liquid pipe 22, adding liquid mixer 23, culture solution filter 24, circulation pipe 25, liquid outlet filter screen 26, circulation pipe valve 27;
[0030] The liquid outlet 12 of the culture tank 1 is located in the middle of the body of the culture tank 1, and is sequentially connected with the culture liquid filter 24 and the additive liquid mixer 23 through the circulation pipe 25;
[0031] The liquid outlet 12 is also provided with a liquid outlet filter 26;
[0032] Adding liquid mixer 23 is a cavity, and adding pipe liquid 22 is connected on the adding liquid mixer 23; It is arranged in parallel with circulation pipe 25;
[0033] The pneumatic circula...
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The invention discloses a plant tissue culture tank which comprises a culture tank, a circulating device out of the tank, a pneumatic circulating device and a gas distributor, wherein the circulating device out of the tank comprises an additive solution mixer and a culture solution filter; the pneumatic circulating device comprises a culture tank connecting part and a pneumatic mixer; the pneumatic mixer is divided into an upper cavity, a middle narrow part and a lower cavity, and a circulating pipe liquid inlet is formed on the side face of the lower cavity; the circulating device out of the tank can be used for filtering a nutritional liquid and solves the problem that the nutritional liquid which is added is not uniformly mixed, and nutritional components can be added in real time. The pneumatic circulating device powered by introduced compressed air does not increase the shearing force, so that the compressed air and the nutritional liquid are mixed with small and uniform bubbles. Moreover, the additive solution and the nutritional liquid are further mixed, so that the mixing effect is relatively good.
Description
technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a plant tissue culture tank. Background technique [0002] my country is one of the countries with the richest medicinal plant resources in the world. With the extensive use of herbal medicines, the excavation and use of medicinal plants far exceeds the growth rate of plants, and the medicinal plant resources are becoming increasingly exhausted. On the one hand, the traditional field cultivation of medicinal plants is affected by "time and place", resulting in large fluctuations in yield and quality. On the other hand, the problem of pesticide residue pollution is serious. The bioreactor culture of plant cells, tissues and organs has the characteristics of rapid growth and easy control, so it can gradually replace field cultivation to meet the needs of the market. [0003] Bioreactors are reactors used for large-scale cultivation of microorganisms, plants and ani...
Claims
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IPC IPC(8): C12M3/00C12M1/12C12M1/04
CPCC12M29/02C12M29/06
Inventor 闫占辉祖慧鹏刘东妮
Owner CHANGCHUN INST OF TECH