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Nucleotide sequence and method used for identifying and differentiating species and varieties of Taxus chinensis

A nucleotide sequence and yew technology, applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve the problems of poor universality of primers, limited test conditions, and high operation requirements

Inactive Publication Date: 2015-07-08
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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AI Technical Summary

Problems solved by technology

RFLP technology is stable and reproducible, but the preparation of probes is cumbersome; RAPD method can reveal the evolutionary relationship between relative species very well, but it is limited by experimental conditions and unstable; ISSR markers are fast, simple and highly polymorphic. Advantages, but its primers have poor versatility, high operating requirements and high cost

Method used

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  • Nucleotide sequence and method used for identifying and differentiating species and varieties of Taxus chinensis
  • Nucleotide sequence and method used for identifying and differentiating species and varieties of Taxus chinensis
  • Nucleotide sequence and method used for identifying and differentiating species and varieties of Taxus chinensis

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Embodiment

[0030] In this example, the rDNA ITS1 sequences of 8 taxus germplasm resources from different provenances were determined, which are respectively: 5 species (and 1 natural mutant line) under the genus Taxus in China, including Taxus dense-leaved (Taxus fuana), Taxus wallichiana var. wallichiana, Taxus wallichiana var. chinensis, Southern yew (Taxus wallichiana var. mairei), Southern yew (Taxus wallichiana var. .mairei yellow), Northeast yew (Taxus cuspidata), and two foreign species, Mandia yew (Taxus x media) and European yew (Taxus baccata). Taxus Amentotaxus argotaenia is an outgroup (GenBank serial number is AF259285.1). The method adopted includes the following specific steps:

[0031] (1) Total DNA extraction

[0032] The leaves, leaf (flower) buds or stems of the plants to be tested were taken, treated with sterile water, ground into powder in liquid nitrogen, and the total DNA was extracted with a QIAGEN kit.

[0033]The specific method is as follows: ① Take 2-3g ti...

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Abstract

The invention discloses a nucleotide sequence used for identifying and differentiating species and varieties of Taxus chinensis. The sequence is originated from an internal transcribed spacer (ITS1) sequence of ribosome DNA (rDNA) of a proto-variety of Taxus wallichiana, and can be used for contrast and identification of the species and varieties of Chinese yew; and through analysis of the genetic characteristics of the germplasm resource of Taxus chinensis at a DNA level, effective molecular marks are provided for differentiation of the species and varieties of Taxus chinensis, and a good base is laid for identification, differentiation and screening of excellent germplasm of the species and varieties of Taxus chinensis. Meanwhile, the invention provides a method for identifying the species of Taxus chinensis by using the nucleotide sequence. The method is simple and practical to operate and can realize convenient, rapid, objective and accurate differentiation of different species and varieties of Taxus chinensis.

Description

technical field [0001] The invention belongs to the technical field of identification of medicinal ornamental plant germplasm resources, and in particular relates to a nucleotide sequence and method for identifying and distinguishing species and varieties of Chinese yew. Background technique [0002] Taxus plants are small evergreen trees or shrubs, dioecious, and are typical negative tree species, and there are basically no pure forests. Because of its good shade tolerance, if it is interplanted under other timber forests, it can not only improve the forest landscape effect, but also improve the comprehensive economic benefits of the forest land. Due to objective factors such as weak population competitiveness, slow natural renewal, and limited geographical distribution, Taxus plants were listed as national second-class protected plants as early as the 1980s. The contradiction between the supply and demand of fir raw materials has become increasingly prominent, and human p...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 王忠徐敏秦亚龙汪仁郑玉红何树兰彭峰
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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