Culture medium for in vitro fast growth of dendrobium officinale

Abstract

The invention provides a culture medium for in vitro fast growth of dendrobium officinale. The culture medium is an MS solid culture medium and further comprises 3-10g / L of rhamnolipid and 2-5g / L of a hydrophilic macromolecular material. By adding the hydrophilic macromolecular material which has a sustained release effect into the culture medium, nutritional substances in the culture medium are promoted to be uniformly distributed, the releasing speed of the nutritional substances is regulated, and lasting and balanced adsorption for the nutritional substances by seedlings is met, so that the growth and development of dendrobium officinale is further promoted. The rhamnolipid which is added into the culture medium has the effects of inhibiting fungi and bacteria, and the pollution on the culture medium can be effectively reduced.

Description

technical field [0001] The invention relates to a culture medium for dendrobium candidum, in particular to a culture medium for rapid growth of dendrobium candidum in vitro. Background technique [0002] Dendrobium officinale is one of the rarest Chinese herbal medicines in my country. It is known as the "gold in medicine". Liver and other health effects. [0003] Wild Dendrobium officinale has strict requirements on the natural ecological conditions, the natural reproduction rate is extremely low, the growth is slow, and the resources are scarce. Due to long-term excavation, the ecological environment has deteriorated, and many rare species are on the verge of extinction. It has been listed as a rare and endangered medicinal plant under national key protection. , which has seriously affected the development and utilization of Dendrobium officinale. [0004] Generally, conventional MS medium is used in the production of Dendrobium candidum. After the seedlings of Dendrobium...

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Problems solved by technology

[0003] Wild Dendrobium officinale has strict requirements on the natural ecological conditions, the natural reproduction rate is extremely low, the growth is slow, and the resources are scarce. Due to long-term excavation, the ecological environment has deteriorated, and many rare species are on the verge of extinction. It has been listed as a rare and endangered medicinal plant under national key protection. , which has seriously affected the development and utilization of Dendrobium officinale

[0004] Generally, conventional MS medium is used in the production of Dendrobium candidum. After the seedlings of Dendrobium candidum are inoculated into the medium, due to the high initial concentration of various nutrients in the medium, the speed of absorption of nutrients by the seedlings is relatively slow. Leaves turn yellow, seedlings shrink, and even cause seedlings to die

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