Diagnostic kit for early liver cancer and use method thereof

A diagnostic kit and early liver cancer technology, applied in the field of biomedicine, can solve the problems of lack of early liver cancer diagnostic markers, etc., and achieve the effect of facilitating large-scale production, reducing the rate of missed diagnosis, and ensuring the accuracy rate

Active Publication Date: 2015-07-29
JIANGSU XIANSIDA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is a lack of sensitive and specific early liver cancer diagnostic markers clinically, and the detected early liver cancer cases account for about 25% of all liver cancer cases

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0011] The method of using the kit of the present invention is to denature the protein through high temperature, release the required oligosaccharide, and add a charged fluorescent group to the end of the oligosaccharide to increase its sensitivity and achieve optional separation. The instrument ABI3500 analyzes and measures the content or fingerprint of the fluorescently labeled N-oligosaccharide chain in the sample (referred to as the G-Test spectrum), analyzes and compares the peak heights of each component of the N-oligosaccharide, and uses the equation to calculate NA3F / (NA2F+ NA2FB) ratio.

[0012] The kit only needs one drop of blood. By using the liver cancer curative effect evaluation technology based on glycoprotein sugar chain detection, it generally only takes 2 or 3 days to accurately detect liver cancer. At the same time, it can accurately detect the liver without liver cancer. , Rapid and convenient detection of the incidence of liver cancer, clear efficacy eval...

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Abstract

The invention relates to a diagnostic kit for early liver cancer. The diagnostic kit comprises the following components of G1: 5% of SDS (sodium dodecyl sulfate); G2: 2.2 microgram/muL glycanase; G3A: 100mM trisulphonate fluorescent marker; G3B: 1M organic matter reducing agent; G4: sialidase; GW: deionized water. The invention also discloses a use method of the diagnostic kit. The diagnostic kit has the advantages that the problem of high missed diagnosing rate of alpha fetoprotein on the liver cancer is solved; the liver cancer can be quickly, simply, conveniently and accurately detected, and the therapy effect of the liver cancer can be tracked.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a diagnostic kit for early liver cancer and a use method thereof. Background technique [0002] HCC is one of the most common malignant tumors in the world, with a high mortality rate due to rapid disease progression, poor prognosis, difficulty in early diagnosis, and limited treatment options. Glycoprotein chains are involved in cell recognition, signal transduction, and immune surveillance, and maintain various orderly life activities in multicellular organisms. Therefore, abnormal changes in sugar chains are usually closely related to the occurrence and development of various diseases, including tumors. [0003] Compared with the various imaging techniques currently used to diagnose liver cancer, such as ultrasonography, computed tomography, and magnetic resonance imaging, these techniques cannot distinguish benign liver changes, such as dysplastic nodules and sclerosing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57438G01N2800/085G01N2800/52G01N2800/7028
Inventor 陈翠英
Owner JIANGSU XIANSIDA BIOTECH CO LTD
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