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A Rhodotorula japonicus strain and its application in the fermentative production of sea red astaxanthin

A technology of Rhododendron glabrata and astaxanthin, which is applied in the field of microorganisms, can solve the problems of inability to meet market demands and high prices, and achieve the effects of good application prospects, economic benefits, high yield and strong growth and fecundity.

Inactive Publication Date: 2018-09-11
WEIHAI LIDA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, many biotechnology companies in the United States, Canada, the European Union and other countries are working on the development and production of such products, but they are far from meeting market demand, so the price is relatively high

Method used

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  • A Rhodotorula japonicus strain and its application in the fermentative production of sea red astaxanthin
  • A Rhodotorula japonicus strain and its application in the fermentative production of sea red astaxanthin

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Embodiment 1

[0023]This embodiment provides the application of Rhodotorula gelatin in the present invention in the fermentation production of sea red astaxanthin, which is realized through the following steps: the described Rhodotorula gelatin is cultured on a slant and seed cultured successively to obtain activated seed liquid; wherein, The slant cultivation temperature is 25° C., and the cultivation time is 60 h. The composition of the slant culture medium is: malt extract powder 130.1 g / L, distilled water 1000 g / L, agar 15 g / L, and the pH is adjusted to 5.5. The seed culture temperature is 25°C, the culture time is 24h h, the shaker speed is 140r / min, the composition of the seed medium is: glucose 10g / L, yeast powder 2g / L, sodium chloride 6g / L, and the remaining ingredients are water, pH5 .5.

[0024] According to the inoculum amount of 2% by volume, the seed solution was inoculated into the fermentation medium containing sea red astaxanthin, and cultured by shaking at 25°C, pH 5.5, 120...

Embodiment 2

[0026] This embodiment provides the application of Rhodotorula gelatin in the present invention in the fermentation production of sea red astaxanthin, which is realized through the following steps: the described Rhodotorula gelatin is cultured on a slant and seed cultured successively to obtain activated seed liquid; wherein, The slant cultivation temperature is 33° C., and the cultivation time is 76 hours. The composition of the slant medium is: malt extract powder 130.1 g / L, distilled water 1000 g / L, agar 20 g / L, and the pH is adjusted to 7.0. The seed culture temperature is 30°C, the culture time is 36h, the shaker speed is 160r / min, the seed medium composition is: glucose 12g / L, yeast powder 3g / L, sodium chloride 9g / L, and the remaining components are water, pH7. 0.

[0027] According to the inoculation amount of 10% by volume, the seed solution was inoculated into the fermentation medium containing sea red astaxanthin, and cultured at 33°C, pH 7.0, and 160 rpm for 76 hour...

Embodiment 3

[0029] This embodiment provides the application of Rhodotorula gelatin in the present invention in the fermentation production of sea red astaxanthin, which is realized through the following steps: the described Rhodotorula gelatin is cultured on a slant and seed cultured successively to obtain activated seed liquid; wherein, The slant cultivation temperature is 26° C., and the cultivation time is 65 hours. The composition of the slant culture medium is: malt extract powder 130.1 g / L, distilled water 1000 g / L, agar 16 g / L, and the pH is adjusted to 5.8. The seed culture temperature is 26°C, the culture time is 28h, the shaker speed is 150r / min, the composition of the seed medium is: glucose 10.5g / L, yeast powder 2.3g / L, sodium chloride 7g / L, and the rest is water. pH5.8.

[0030] According to the inoculation amount of 3% by volume, the seed solution was inoculated into the fermentation medium containing sea red astaxanthin, and cultured by shaking at 28°C, pH 6.0, 130rpm for 6...

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Abstract

This application discloses a strain of rhodotorula mucilaginosa GH101, and the invention also discloses the application of said rhodotorula mucilaginosa in the fermentation production of sea red astaxanthin, which is realized by the following steps: the rhodotorula mucilaginosa is successively Carry out slant culture and seed culture to obtain activated seed liquid; inoculate the seed liquid into the sea red astaxanthin-containing fermentation medium according to the volume percentage of 2-10% inoculum, at 25-33 ° C, pH5.5-7.0, 120-160rpm shaking culture for 60-76h, to obtain Rhodotorula colloides thallus containing sea red astaxanthin. The rhodotorula japonicum provided by the invention can be used to ferment and produce sea red astaxanthin. The rhodotorula gelatin is used to produce sea red astaxanthin, and the production strains are repeatedly screened to obtain a strain with strong growth and fecundity and high sea red astaxanthin output. Higher strains have good application prospects for the industrial production of astaxanthin.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a strain of rhodotorula colloides and its application in fermenting and producing sea red astaxanthin. Background technique [0002] Sea red astaxanthin is a kind of carotenoid with strong anti-oxidation ability and biological coloring ability. It has been widely used in food, medicine, cosmetics, feed and many other fields. It has high application value in preventing cardiovascular and cerebrovascular diseases, protecting eyes and central nervous system, preventing ultraviolet radiation, inhibiting tumors, anti-inflammation, and relieving exercise fatigue. Sea red astaxanthin, a carotenoid with high antioxidant capacity, its mobility is close to natural astaxanthin ester in thin layer chromatography, and its antioxidant capacity is also close to natural L-astaxanthin, so it is named, DPPH Its antioxidant capacity is about 20% to 40% stronger than that of natural L-astaxanthin....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12P23/00
CPCC12P23/00C12N1/145C12R2001/645
Inventor 魏升宁张文丽孙常明程振伟张美丽李纪丰
Owner WEIHAI LIDA BIOTECH