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Pediococcus pentosaceus strain and application thereof

A technology of Pediococcus pentosaceae and strains, applied in the direction of bacteria, microorganisms, microorganisms, etc., to achieve the effect of easy cultivation and preservation, and good growth

Active Publication Date: 2015-08-12
连云港恺骐食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

β-glucosidase from different microbial sources has substrate selectivity. The β-glucosidase produced by microorganisms is a component of the cellulase system. Can the β-glucosidase that hydrolyzes cellulose hydrolyze sugar in plants? Conjugates, systematic studies on this aspect have not been reported

Method used

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  • Pediococcus pentosaceus strain and application thereof
  • Pediococcus pentosaceus strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] This example illustrates that Pediococcus pentosacea ( Pediococcus pentosaceus ) Screening, purification and identification methods of CS1406.

[0029] 1. Breeding of strains

[0030] The naturally fermented whole wheat dough used for strain screening was collected from Shandong, Henan and other places. Weigh 25 g of the sample, put it into a Erlenmeyer flask containing 225 mL of wort under sterile conditions, and enrich and cultivate at 25-28 °C for 24 h. Draw 1 mL of culture solution for gradient dilution, spread the dilution evenly on the solidified Elliker separation medium, and incubate at 35-37°C for 3-4 days, pick the colonies visible to the naked eye in the medium, and further spread on On the MRS solid medium, continue to incubate at 35-37°C for 2-3 days until a single colony is obtained, and then transfer to the MRS solid slant preservation medium. The final concentration of the Elliker separation medium consists of: tryptone 20.0g / L, yeast extract 5.0g / L, ...

Embodiment 2

[0036] This example illustrates that Pediococcus pentosacea ( Pediococcus pentosaceus ) Cultivation of CS1406 and application in biotransformation to prepare 2,6-dimethoxy-p-benzoquinone.

[0037] Pick 1 ring of bacteria from the MRS slant medium and insert it into Elliker liquid medium, and culture it statically at 37°C for 2 days, then transfer the seed culture solution into the fermentation medium at 5% inoculum size, and ferment for 4 days at 37°C under static conditions . The fermentation broth was centrifuged, and the precipitate was discarded. The supernatant was extracted three times with 50 ml of chloroform, and the extract was evaporated and concentrated under reduced pressure to obtain crude 2,6-dimethoxy-p-benzoquinone. The concentrate was then dissolved in 5ml of methanol, filtered through a 0.22μm filter membrane, and the amount of 2,6-dimethoxy-p-benzoquinone produced in the fermentation product was determined to be 893.0μg / g by high performance liquid chromato...

Embodiment 3

[0039] This example illustrates that Pediococcus pentosacea ( Pediococcus pentosaceus ) Cultivation of CS1406 and application in biotransformation to prepare 2,6-dimethoxy-p-benzoquinone.

[0040] Pick 1 ring of bacteria from the MRS slant medium and insert it into Elliker liquid medium, and culture it statically for 2 days at 37°C. Put the seed culture solution into the fermentation medium at 10% inoculum size, and ferment for 3 days at 35°C under static conditions. . The fermentation broth was centrifuged, and the precipitate was discarded. The supernatant was extracted three times with 50 ml of chloroform, and the extract was evaporated and concentrated under reduced pressure to obtain crude 2,6-dimethoxy-p-benzoquinone. The concentrate was then dissolved in 5ml of methanol, filtered through a 0.22μm filter membrane, and the amount of 2,6-dimethoxy-p-benzoquinone in the fermentation product was determined to be 942.1μg / g by high performance liquid chromatography.

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Abstract

The invention discloses a Pediococcus pentosaceus strain and application thereof. The collection number of the Pediococcus pentosaceus CS1406 is CCTCC NO:M2015169. The strain can be fermented or the free cell of the strain can biotransform para-benzoquinone glucoside in the wheat embryo, thereby producing the 2,6-dimethoxy para-benzoquinone. The Pediococcus pentosaceus CS1406 is derived from the traditional natural fermentation food, and belongs to a microbe generally recognized as safe. The strain can be easily cultured and preserved. The 2,6-dimethoxy para-benzoquinone obtained by wheat embryo fermentation is 942.1 mu g / g. The Pediococcus pentosaceus strain provides a new way for enhancing bioavailability of the wheat embryo, has important application prospects.

Description

technical field [0001] The present invention relates to a Pediococcus pentosaceae CS1406 ( Pediococcus pentosaceus CS1406) strain, and its application in biotransformation of wheat germ to produce 2,6-dimethoxy-p-benzoquinone, belongs to the technology in the field of industrial microorganisms. Background technique [0002] Quinone compounds refer to a class of natural pigment organic compounds with an unsaturated cyclic diketone structure in the molecule, and are mainly divided into four types: benzoquinone, naphthoquinone, phenanthrenequinone and anthraquinone. Quinone compounds have biological activities such as diarrhea, antibacterial, hemostasis, expansion of coronary arteries, deworming, and spasm relief. They are widely found in Polygonaceae, Rubiaceae, Fabaceae, Liliaceae Aloe, Lamiaceae and some lower plants. The research results show that 1,4-p-benzoquinone is a compound with broad-spectrum antibacterial activity, which can kill a variety of pathogenic bacteria a...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/66C12R1/01
CPCC12P7/66C12N1/205C12R2001/01
Inventor 王立梅齐斌朱益波郑丽雪朱颖越梁剑光姚海霞丁树慧陈建
Owner 连云港恺骐食品有限公司
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