Pediococcus pentosaceus strain and application thereof
A technology of Pediococcus pentosaceae and strains, applied in the direction of bacteria, microorganisms, microorganisms, etc., to achieve the effect of easy cultivation and preservation, and good growth
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Embodiment 1
[0028] This example illustrates that Pediococcus pentosacea ( Pediococcus pentosaceus ) Screening, purification and identification methods of CS1406.
[0029] 1. Breeding of strains
[0030] The naturally fermented whole wheat dough used for strain screening was collected from Shandong, Henan and other places. Weigh 25 g of the sample, put it into a Erlenmeyer flask containing 225 mL of wort under sterile conditions, and enrich and cultivate at 25-28 °C for 24 h. Draw 1 mL of culture solution for gradient dilution, spread the dilution evenly on the solidified Elliker separation medium, and incubate at 35-37°C for 3-4 days, pick the colonies visible to the naked eye in the medium, and further spread on On the MRS solid medium, continue to incubate at 35-37°C for 2-3 days until a single colony is obtained, and then transfer to the MRS solid slant preservation medium. The final concentration of the Elliker separation medium consists of: tryptone 20.0g / L, yeast extract 5.0g / L, ...
Embodiment 2
[0036] This example illustrates that Pediococcus pentosacea ( Pediococcus pentosaceus ) Cultivation of CS1406 and application in biotransformation to prepare 2,6-dimethoxy-p-benzoquinone.
[0037] Pick 1 ring of bacteria from the MRS slant medium and insert it into Elliker liquid medium, and culture it statically at 37°C for 2 days, then transfer the seed culture solution into the fermentation medium at 5% inoculum size, and ferment for 4 days at 37°C under static conditions . The fermentation broth was centrifuged, and the precipitate was discarded. The supernatant was extracted three times with 50 ml of chloroform, and the extract was evaporated and concentrated under reduced pressure to obtain crude 2,6-dimethoxy-p-benzoquinone. The concentrate was then dissolved in 5ml of methanol, filtered through a 0.22μm filter membrane, and the amount of 2,6-dimethoxy-p-benzoquinone produced in the fermentation product was determined to be 893.0μg / g by high performance liquid chromato...
Embodiment 3
[0039] This example illustrates that Pediococcus pentosacea ( Pediococcus pentosaceus ) Cultivation of CS1406 and application in biotransformation to prepare 2,6-dimethoxy-p-benzoquinone.
[0040] Pick 1 ring of bacteria from the MRS slant medium and insert it into Elliker liquid medium, and culture it statically for 2 days at 37°C. Put the seed culture solution into the fermentation medium at 10% inoculum size, and ferment for 3 days at 35°C under static conditions. . The fermentation broth was centrifuged, and the precipitate was discarded. The supernatant was extracted three times with 50 ml of chloroform, and the extract was evaporated and concentrated under reduced pressure to obtain crude 2,6-dimethoxy-p-benzoquinone. The concentrate was then dissolved in 5ml of methanol, filtered through a 0.22μm filter membrane, and the amount of 2,6-dimethoxy-p-benzoquinone in the fermentation product was determined to be 942.1μg / g by high performance liquid chromatography.
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