Method for developing SSR molecular markers on large scale

Abstract

The invention relates to a method for developing SSR molecular markers on a large scale. The method for developing the SSR molecular markers on the large scale comprises the following steps: firstly, acquiring a genomic sequencing sequence or an EST sequence on GenBank; secondly, acquiring SSR locus information of the genomic sequencing sequence or the EST sequence on the GenBank; thirdly, classifying SSR locus according to SSR sequence motif types; fourthly, carrying out reverse complementary treatment on SSR locus information of a database 3.CSV; fifthly, acquiring SSR molecular markers and upstream and downstream sequences in a database 4.CSV and a database 5.CSV by adopting SSR locus sequence interception software, and creating a sequence database according to the sequence motif types; and sixthly, screening repeated or similar SSR locus in CSV files of SSRs of the same type. The method for developing the SSR molecular markers on the large scale has the advantages that SSR marker locus in a genome can be located, SSR sequence fragments can be intercepted on the large scale, repeatability analysis can be carried out on different SSR sequences, and SSR molecular marker development efficiency is greatly improved.

Description

technical field [0001] The invention belongs to the fields of molecular biology and bioinformatics, and relates to a method for developing SSR molecular markers in large quantities. Background technique [0002] Molecular markers are genetic markers based on the polymorphism of the genetic material nucleic acid of an organism. They have the characteristics of abundant quantity, stable genetics, no restriction on gene expression, and easy operation. Molecular markers can be used to directly detect genome Genetic Variation. It can accurately reveal individual differences among different species, varieties, varieties, and strains of the same species from the level of genetic material DNA, and has unparalleled advantages. Molecular markers have been widely used in the construction of genetic maps and gene mapping, and are considered to be a useful tool for identifying varieties and strains (including hybrids and inbred lines) and analyzing the genetic diversity of germplasm res...

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Problems solved by technology

[0007] Purpose of the invention: The present invention makes improvements to the problems existing in the above-mentioned prior art, that is, the present invention discloses a method for developing SSR molecular markers in large quantities, which can solve the repetitiveness of SSR marker development caused by a large number of highly repetitive segments of the genome The problem of high efficiency and low effectiveness can greatly improve the development efficiency of SSR molecular markers, and the developed SSR molecular markers have good polymorphism, saving a lot of manpower and material resources

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