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A preparation method of hydroxyapatite multilevel composite microspheres

A technology of hydroxyapatite and composite microspheres, which is applied in medical science, bulk transportation, prostheses, etc., to achieve the effects of uniform particle size distribution, cheap raw materials, and simple operation

Inactive Publication Date: 2017-08-18
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved in the present invention is to overcome the deficiencies of the existing artificially synthesized hydroxyapatite technology, and provide a preparation method of hydroxyapatite multi-level composite microspheres

Method used

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  • A preparation method of hydroxyapatite multilevel composite microspheres
  • A preparation method of hydroxyapatite multilevel composite microspheres
  • A preparation method of hydroxyapatite multilevel composite microspheres

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Dissolve 0.08g of casein in 20ml of sodium carbonate solution (100mM), slowly add 20ml of calcium chloride solution (100mM) into the sodium carbonate solution containing casein at a rate of 1ml / min, after the dropwise addition, stir at room temperature In 20 minutes, the mother liquor of calcium carbonate microspheres containing casein was obtained.

[0038] Configure 40ml of 30mM disodium hydrogen phosphate solution, add the above-mentioned disodium hydrogen phosphate solution dropwise to the freshly generated calcium carbonate microsphere mother liquor containing casein at a rate of 2ml / min, and adjust the pH value to 10. React for 0.5-1.5 h under stirring at 600 rpm, wash and dry the precipitate to obtain hydroxyapatite multi-level composite microspheres.

Embodiment 3

[0043] Apply the method in Example 1 to prepare casein-containing calcium carbonate microsphere mother liquor.

[0044] Centrifuge and precipitate the casein-containing calcium carbonate microsphere mother liquor, freeze-dry to obtain calcium carbonate microspheres, resuspend 0.1 g of casein-containing calcium carbonate microspheres in 10 ml deionized water, and obtain calcium carbonate microspheres For resuspension, configure 50ml of disodium hydrogen phosphate solution (500mM), add this disodium hydrogen phosphate solution to the resuspension of calcium carbonate microspheres, stir at 100rpm for 0.5h, after the reaction is over, wash with deionized water, centrifuge, Freeze-dry to obtain hydroxyapatite multi-level composite microspheres.

[0045] The prepared hydroxyapatite multi-level composite microspheres were detected by FTIR, and FTIR showed PO 4 3- , CO 32- and the strong absorption peak of casein, PO 4 3- peak with CO 3 2- The peak intensities are close to each...

Embodiment 4

[0049] Apply the method in Example 1 to prepare casein-containing calcium carbonate microsphere mother liquor.

[0050] Centrifuge and precipitate the mother liquor of calcium carbonate microspheres containing casein, freeze-dry to obtain calcium carbonate microspheres, resuspend 0.2 g of calcium carbonate microspheres containing casein in water to obtain resuspended calcium carbonate microspheres solution, configure 40ml of 30mM disodium hydrogen phosphate solution, add the disodium hydrogen phosphate solution dropwise into the calcium carbonate microsphere resuspension at a rate of 2ml / min, adjust the pH value to 10, and stir at 60°C and 600rpm After reacting for 1 hour, the precipitate was washed and dried to obtain hydroxyapatite multi-level composite microspheres.

[0051] The prepared hydroxyapatite multi-level composite microspheres were detected by FTIR, and FTIR showed PO 4 3- peak, CO 3 2- The peak of casein and the peak of casein, compare embodiment 3, PO 4 3-...

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Abstract

The invention provides a preparation method of hydroxyapatite multi-stage composite microspheres, comprising firstly dissolving casein in sodium carbonate solution, then slowly adding calcium chloride solution into the above sodium carbonate solution, and stirring Obtain the mother liquor of calcium carbonate microspheres containing casein, directly mix the mother liquor with disodium hydrogen phosphate solid powder or solution, or centrifuge and precipitate the mother liquor of calcium carbonate microspheres containing casein, freeze-dry to obtain calcium carbonate microspheres, The calcium carbonate microspheres are resuspended to obtain calcium carbonate microsphere suspension, and then mixed with disodium hydrogen phosphate solution, the pH value of the obtained mixture is adjusted, stirred, centrifuged and dried to obtain hydroxyapatite multi-level composite microspheres. The preparation method of the present invention has cheap raw materials, wide sources, and simple operation. The obtained microspheres have a uniform particle size distribution and the surface of the microspheres is formed by mutual cross-linking of flaky, needle-shaped or rod-shaped crystals, which is beneficial to the repair of bone defects and has great advantages. application prospects.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and more specifically relates to a preparation method of hydroxyapatite multilevel composite microspheres. Background technique [0002] At present, bone defect is a common clinical disease. According to statistics, there are more than 1 million patients with bone defect caused by disease, trauma, tumor, etc. in my country every year. Repairing large-scale bone defect is still one of the difficult problems in orthopedic treatment. The treatment methods for bone defects usually include bone grafting, artificial bone grafting substitute materials, etc., and bone grafting includes autologous bone, allogeneic bone and xenograft bone grafting. However, the source of autologous bone is limited, and it will cause new defects in the donor site. The osteoinductive effect of allogeneic bone graft is poor, and immune rejection is prone to occur. Therefore, it is a research hotspot to obtain an i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/16A61L27/40A61L27/12A61L27/50A61L27/54A61L27/58
Inventor 李燕马双双曹智楠龚逸鸿王丹丹钟惠湘
Owner SUN YAT SEN UNIV