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Lactobacillus strain and primer pair thereof

A technology of lactic acid bacteria strains and primer sets, applied in the direction of bacteria, microorganisms, recombinant DNA technology, etc., can solve the problem of lack of DNA molecular identification markers for strain identification

Inactive Publication Date: 2015-08-26
萧夏青
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although L.paracasei subsp.paracasei NTU 101 has been successfully commercialized, it still lacks DNA molecular identification markers required for strain identification and patent protection

Method used

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  • Lactobacillus strain and primer pair thereof
  • Lactobacillus strain and primer pair thereof
  • Lactobacillus strain and primer pair thereof

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Embodiment Construction

[0033] In order to more clearly describe a lactic acid bacteria strain and its primer set proposed by the present invention, preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0034]NTU 101 lactic acid bacteria strain (Lactobacillus mutant), an excellent native lactic acid bacteria strain - Lactobacillus paracasei subsp.paracasei NTU 101 developed by Professor Pan Ziming, director of the Institute of Microbiology and Biochemistry, National Taiwan University, and his R&D team, was established in 2013. It was deposited at the German Culture Collection of Microorganisms (Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH (DSMZ, Inhoffenstr. 7B, D-38124 Braunschweig, Germany)) on November 18, and the deposit number is DSM 28047. The name of the unit that preserves the sample of the biological material: DSMZ-German Culture Collection of Microorganisms, address: 7B, Inn and Finnstadt Street, Braunsc...

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Abstract

The present invention discloses a lactobacillus strain and a primer pair thereof, wherein the lactobacillus strain is Lactobacillus paracasei subsp.paracasei NTU 101 stored in DSMZ the storage number is DSM 28047, and the Lactobacillus paracasei subsp.paracasei NTU 101 has a nucleotide sequence of SEQ ID NO:1. The present invention provides the nucleotide sequence of the Lactobacillus paracasei subsp.paracasei NTU 101 and the primer pair thereof, so that relevant technical staff can have basis on strain identification for the Lactobacillus paracasei subsp.paracasei NTU 101; in addition, the relevant technical staff also can quickly identify the NTU 101 strain through DNA molecular recognition marks without cultivating an isolated strain or cultivating viable bacteria.

Description

technical field [0001] The invention relates to a lactic acid bacterial strain, especially a lactic acid bacterial strain related to Lactobacillus paracasei subsp. paracasei NTU 101 and a primer set thereof. Background technique [0002] Lactic acid bacteria refer to bacteria that can metabolize carbohydrates and produce more than 50% lactic acid, such as Lactobacillus, Streptococcus, and Leuconostoc. Humans have a long history of drinking fermented milk products. Therefore, lactic acid bacteria have always been considered as very safe bacteria (GRAS, generally recognized as safe) and are the most representative intestinal beneficial bacteria. [0003] Lactic acid bacteria are the most important group of probiotics. Probiotics are defined as one or more microorganisms that can improve the quality of human intestinal flora when fed to humans. Among them, the mechanism by which lactic acid bacteria can improve the quality of intestinal flora is as follows: [0004] (1) Produ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/68C12Q1/04C12N15/11C12R1/225
Inventor 潘子明萧夏青
Owner 萧夏青