Use of FAM135B inhibitor in treatment and/or prevention of esophageal squamous cancer

A FAM135B and inhibitor technology, applied in gene therapy, DNA/RNA fragments, recombinant DNA technology, etc., can solve problems such as no related reports

Active Publication Date: 2015-09-02
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] FAM135B is located on chromosome 8, and its protein contains 1,406 amino acids. The Genbank accession number of the FAM135B gene on NCBI is Gene ID: 51059. There is no relevant report on its function in the literature

Method used

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  • Use of FAM135B inhibitor in treatment and/or prevention of esophageal squamous cancer
  • Use of FAM135B inhibitor in treatment and/or prevention of esophageal squamous cancer
  • Use of FAM135B inhibitor in treatment and/or prevention of esophageal squamous cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: the method for detecting the expression of FAM135B gene

[0039] 1. Sample Total RNA Extraction – Trizol method to extract total RNA from tissues and cells

[0040] (1) Sample processing (bacteria and cells do not need to be ground);

[0041] Put 1cm in aluminum foil 2 Tissue samples of about the size are crushed, transferred to an Eppendorf tube with steel balls added, and grinded with a grinder (30 1 / s, 8min)

[0042] Note: This step should be operated in a low-temperature liquid nitrogen environment as much as possible, and bacteria and cell samples do not need to be ground.

[0043] (2) Add 1ml Trizol to the ground Eppendorf tube, shake and mix;

[0044] (3) Transfer the mixed solution to a new Eppendorf tube, add 200 μl chloroform, shake and mix;

[0045] (4) Centrifugation: 4°C, 12000rpm, 15min;

[0046] (5) Transfer the centrifuged supernatant to a new Eppendorf tube, add 500 μl isopropanol, mix gently, and let stand at room temperature for 15...

Embodiment 2

[0080] Example 2: The effect of FAM135B gene alteration on the prognosis of patients with esophageal cancer

[0081] By analyzing the genomes of patients with esophageal cancer in Chaoshan area of ​​Guangdong Province, using the Kaplan-Meier analysis method in SPSS statistical software, it was found that there were non-synonymous mutations and amplifications, that is, missense mutations (missense) and amplification changes at the DNA level . FAM135B alterations in FAM135B decreased the overall survival of patients compared with FAM135B wild-type patients, with a statistically significant difference, pfigure 1 ).

Embodiment 3

[0082] Example 3: Expression of FAM135B in esophageal squamous cell carcinoma cell lines and normal esophageal cells

[0083] The expression of FAM135B gene in esophageal squamous cell carcinoma cell lines and normal esophageal cells was measured by real-time PCR method (see figure 2 ). Among them, the abscissa represents the human esophageal cancer cell line. The expression of FAM135B gene was increased in the esophageal cancer cell line compared with the normal cell line NE3.

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Abstract

The invention relates to the use of an FAM135B inhibitor in treatment and/or prevention of the esophageal squamous cancer. Further the invention relates to the use of the FAM135B genome mutation in diagnosis and/or prognosis of the esophageal squamous cancer.

Description

technical field [0001] The present invention relates to the technical field of FAM135B inhibitor in the treatment and / or prevention of esophageal squamous cell carcinoma. On the other hand, the present invention relates to the technical field of FAM135B gene mutation in the diagnosis and / or prognosis of esophageal squamous cell carcinoma. Background technique [0002] Esophageal cancer is a common digestive tract tumor. About 300,000 people die from esophageal cancer every year in the world, and it is the sixth leading cause of cancer death in the world. See Kamangar, F., G.M.Dores, et al. (2006). "Patterns of cancer incidence, mortality, and prevalence across five continents: defining priorities to reduce cancer disparities in different geographic regions of the world." J Clin Oncol 24(14):2137-2150. About 70% of esophageal cancer in the world occurs in China, and 90% of esophageal cancer in China is esophageal squamous cell carcinoma. So far, little is known about the me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K39/395A61K48/00C12Q1/68C12N15/11A61P35/00
Inventor 詹启敏欧云尉宋咏梅付明马小娟
Owner CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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