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A kit to test the effectiveness of oxaliplatin for colorectal cancer

A colorectal cancer and oxaliplatin technology, applied in the field of kits for detecting the effectiveness of oxaliplatin for colorectal cancer, can solve the problems of lack of clinical verification of oxaliplatin sensitivity and drug resistance.

Active Publication Date: 2018-01-09
SHANGHAI YANGPU CENT HOSPITAL
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Problems solved by technology

However, the three-year disease-free survival rate of patients with stage III colorectal cancer receiving adjuvant chemotherapy containing oxaliplatin is still only 65% ​​(Uncu D, Aksoy S, Cetin B, et al. Results of adjuvant FOLFOX regimens in stage III colorectal cancer patients: retrospective analysis of 667 patients. Oncology. 2013; 84(4):240-245)
An effective treatment strategy is to distinguish the patient groups who may benefit more or less from oxaliplatin adjuvant chemotherapy, and individualize drug therapy for CRC patients, but there is still a lack of predictive markers for oxaliplatin sensitivity. Clinical validation of sex and drug resistance

Method used

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  • A kit to test the effectiveness of oxaliplatin for colorectal cancer
  • A kit to test the effectiveness of oxaliplatin for colorectal cancer
  • A kit to test the effectiveness of oxaliplatin for colorectal cancer

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Embodiment Construction

[0017] The kit includes: a real-time fluorescence quantitative PCR amplification system composed of 2×SYBR Green qPCR Mix, ultrapure water and two pairs of qPCR detection primers (see Table 1).

[0018] Table 1 Three pairs of qPCR detection primers

[0019]

[0020]

[0021] For the real-time fluorescent quantitative PCR amplification system, the final concentration of each 20 μL reaction system is: forward and reverse primers 4uM each, 2×SYBR Green qPCR Mix 10 μL, and ultrapure water to make up to 20uL.

[0022] The mM and μM are units of molar concentration, referring to the number of moles of solute per liter of solution.

[0023] The present invention detects the change of MAP4K1, PIK3CA gene copy number and predicts the method for rectal cancer chemotherapy curative effect, mainly comprises the following steps:

[0024] (1) Collect surgically resected cancer tissue samples from patients with colorectal cancer and embed them in paraffin, and follow up the recurrence...

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Abstract

The invention relates to a kit for detecting effectiveness of oxaliplatin to colorectal cancer. The kit comprises a pair of amplification primer sequences for detecting MAP4K1 gene copy number, a pair of amplification primer sequences for detecting PIK3CA gene copy number and a pair of primer sequences for detecting reference gene GAPDH gene copy number. Through the kit designed in the invention, effectiveness of oxaliplatin to colorectal cancer can be detected rapidly and effectively, and whether a patient to be detected is suitable to use oxaliplatin as a first-line chemotherapy drug is evaluated. The kit provided by the invention provides effective reference for clinical medication.

Description

[technical field] [0001] The invention relates to a kit for detecting the effectiveness of oxaliplatin for colorectal cancer. [Background technique] [0002] Colorectal cancer (CRC) is the third most common malignant tumor in the world and the second leading cause of death caused by cancer, which seriously threatens human health (Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Globalcancer statistics. CA: a cancer journal for clinicians. 2011; 61(2):69-90.). The clinical outcome of colorectal cancer has improved dramatically in recent decades, not only due to advances in surgical techniques but also to interventional therapies with chemotherapy and targeted agents (Moertel CG, Fleming TR, Macdonald JS, et al. Levamisole and fluorouracil for adjuvant therapy of resected colon carcinoma. The New England journal of medicine. 1990; 322(6): 352-358; Andre T, Boni C, Navarro M, et al. Improved overall survival with oxaliplatin, fluorouracil, and leucovorin therapy as adju...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/106
Inventor 李华光林谋斌汤二将嵇承栋尹路李阿健江慧红
Owner SHANGHAI YANGPU CENT HOSPITAL
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