Extracting method and application of cigarette-used extract from termitornyces albuminosus fermentation substances

An extraction method and a technology of Fructus Gallus chinensis are applied in the field of preparation of flavors and fragrances for tobacco, and achieve the effects of natural fragrance, low cost and simple process

Inactive Publication Date: 2015-09-09
HUBEI CHINA TOBACCO IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report about the use of the fermented product extract of the submerged fermentation of Gallus fruticosa for tobacco additives

Method used

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  • Extracting method and application of cigarette-used extract from termitornyces albuminosus fermentation substances
  • Extracting method and application of cigarette-used extract from termitornyces albuminosus fermentation substances
  • Extracting method and application of cigarette-used extract from termitornyces albuminosus fermentation substances

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Cut small pieces of tissue fragments from the stipe of the sporocarp of Gallinarum albumen under aseptic conditions, inoculate them on the separation agar plate, and cultivate them statically at a temperature of 28°C for 7 days. Pick 5 pieces of agar with mycelia with an inoculation hook under the condition of bacteria, inoculate them into the seed medium, and cultivate them for 7 days at a temperature of 25° C. and a rotation speed of 150 r / min to obtain an inoculum of Gallus firtrii. According to volume ratio 15%, the inoculum of Gallus fruticosa is inoculated in the fermentation medium, at a temperature of 25°C, the ventilation rate is 1.0dm 3 / min, under the condition of rotating speed 100r / min, cultivated for 4 days, carried out submerged fermentation to obtain the fermented mixture of Gallus firsum;

[0021] (2) steam distilling and extracting the volatile oil from the fermented mixture of the fermented fermented bacteria in the step (1) to obtain a light red ...

Embodiment 2

[0025] (1) Cut small pieces of tissue fragments from the stipe of the sporocarp of Gallinarum albumen under aseptic conditions, inoculate them on the separation agar plate, and cultivate them statically at a temperature of 30° C. for 5 days. 10 pieces of agar with hyphae were picked with an inoculation hook under the condition of bacteria, inoculated into the seed medium, and cultivated for 5 days at a temperature of 28° C. and a rotation speed of 160 r / min to obtain the inoculum of Gallus fruticosa. Inoculate the inoculum of Gallus pilosulae in the fermentation medium at 10% by volume, at a temperature of 30°C, the aeration rate is 1.2dm 3 / min, under the condition of rotating speed 150r / min, cultivated for 5 days, carried out submerged fermentation to obtain the fermented mixture of Gallus fruticosa;

[0026] (2) steam distilling and extracting the volatile oil from the fermented mixture of the fermented fermented bacteria in the step (1) to obtain a light red transparent vo...

Embodiment 3

[0030] (1) Cut the small tissue fragments from the stipe of the sporocarp of Gallinarum albumen under aseptic conditions, inoculate them on the separation agar plate, and cultivate them statically at a temperature of 29° C. for 6 days. 15 pieces of agar with hyphae were picked with an inoculation hook under the condition of bacteria, inoculated into the seed medium, and cultivated for 3 days at a temperature of 30° C. and a rotation speed of 100 r / min to obtain an inoculum of Gallus firtrii. Inoculate the inoculum of Gallus pilosulae in the fermentation medium by 5% by volume, at a temperature of 20°C, the ventilation rate is 0.8dm 3 / min, under the condition of rotating speed 120r / min, cultivated for 3 days, and carried out submerged fermentation to obtain the fermented mixture of Gallus fruticosa;

[0031] (2) steam distilling and extracting the volatile oil from the fermented mixture of the fermented fermented bacteria in the step (1) to obtain a light red transparent volat...

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Abstract

The invention provides an extracting method of cigarette-used extract from termitornyces albuminosus fermentation substances. The method comprises steps as follows: (1), a termitornyces albuminosus fruiting body is inoculated, a termitornyces albuminosus inoculum is obtained and subjected to submerged fermentation, and a termitornyces albuminosus fermented mixture is obtained; (2), the termitornyces albuminosus fermented mixture in the step (1) is subjected to steam distillation, and volatile oil is obtained; (3), the volatile oil obtained from the step (2) is purified, and the cigarette-used extract from the termitornyces albuminosus fermentation substances is obtained. The submerged fermentation substances of termitornyces albuminosus are extracted and used in flavors and essences for cigarettes, the cigarette-used extract is natural, non-toxic, stable in yield and high in controllability and is not affected by seasons, climate, diseases and other factors, the production period is short, and the cost is low. Meanwhile, the prepared flavors and essences for the cigarettes have more natural and lasting fragrance and better harmony and can cooperate with other flavors and essences for the cigarettes to increase the fragrance. The process is simple, the cost is low, and high practicability is realized.

Description

technical field [0001] The invention relates to the field of preparation of flavor and fragrance for tobacco, in particular to an extraction method and application of an extract of fermented product of Gallus pilosulae for tobacco. Background technique [0002] Termitornyces albuminosus, also known as umbrella mushroom, belongs to Basidiomycetes, Agaricaceae, Pleurotaceae, and Gallus genus. It is a precious edible fungus with pleasant aroma, rich nutrition and certain health care functions. Distributed in my country's Yunnan, Sichuan, Guizhou, Hunan, Hubei, Taiwan and Hong Kong. Gallium fruticosa is rich in saponins, cerebrosides, cellulase, polysaccharides, polyphenols and other active ingredients, which have biological activities such as refreshing, analgesic, antiviral, anti-inflammatory, anti-tumor and antioxidant. At present, the research and application of Gallus pilosula mainly focus on edible and medicinal aspects, and the extraction and application of its fermentati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02A24B3/12C12R1/645
Inventor 郭国宁刘冰岳海波
Owner HUBEI CHINA TOBACCO IND
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